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Establishment of hTERT immortalized mouse cardiosphere derived cell line

ABSTRACT: Establishment of hTERT immortalized mouse cardiosphere derived cell line

PROVIDER: PRJNA544715 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9121340_1.fastq.gz	Fastqsanger.gz
	SRR9121340_2.fastq.gz	Fastqsanger.gz
	SRR9121341_1.fastq.gz	Fastqsanger.gz
	SRR9121341_2.fastq.gz	Fastqsanger.gz
	SRR9121342_1.fastq.gz	Fastqsanger.gz

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Establishment of hTERT immortalized mouse cardiosphere derived cell line.

Project description:Establishment of hTERT immortalized mouse cardiosphere derived cell line.

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Establishment of hTERT immortalized mouse cardiosphere derived cell line

Project description:The analysis showed differences of transcriptomes between primary CDCs and immortalized CDCs as well as revealed TGF-β inhibitor-SB431542 treatment induced expression changes. We identified hTERT immortalization could result the cell proliferation and cell cycle related genes upregulation in iCDC. KEGG results demonstrated that TGF-β inhibitor SB431542 treatment could inhibit cardiomyopathy-related pathway which indicate TGF-β signaling pathway may serve as new target for cardiomyopathy therapy, and iCDC could be used as a heart-related chemicals screening.

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Establishment of hTERT immortalized mouse cardiosphere derived cell line.

Project description:The analysis showed differences of transcriptomes between 5-azacytidine induced CDCs and control CDCs. We identified 10uM 5-azacytidine treatment for 3 days, following with normal culture for 4 weeks,could induce hTERT immortlized CDCs cardiac differentiation. Differential gene expression cluster analysis indicated big difference after 5-aza treatment, which include cardiomyocyte specific genes,such as tnnt2,desmin,mef2a,etc.

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Expression data for hTERT-immortalized human Sertoli cell line versus original primary human Sertoli cells

Project description:Primary human Sertoli cells (hSerCs) were immortalized by transducing human TERT into the genome to produce human TERT-immortalized human Sertoli cells (hTERT hSerCs). Total RNA was isolated from both primary hSerCs and hTERT hSerCs and submitted for Affymetrix gene analysis. Gene expression differences of common Sertoli cell marker genes and xenobiotic transporters was preferentially assessed between each cell type.

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Comparison of the transcriptomes of hTERT-immortalized and wild type marmoset fibroblasts by next-generation RNA sequencing.

Project description:Neuborn marmoset fibroblasts were immortalized by piggyBac transposition of hTERT expression cassette. The gene expression of the resulting one polyclonal and two monoclonal immortalized cell lines were then compared with the wild type cells used for their derivation.

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Expression data for hTERT-immortalized human Sertoli cell line versus original primary human Sertoli cells

Project description:Expression data for hTERT-immortalized human Sertoli cell line versus original primary human Sertoli cells

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Genetic profiling of fetal human hepatocytes immortalized with hTERT

Project description:Genetic profiling of fetal human hepatocytes immortalized with hTERT

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HOXA9 transcriptomic analysis in GBM cell lines (U87MG, U251 and primary GBML18) and in immortalized human astrocytes (hTERT/E6/E7)

Project description:Assess gene expression patterns upon HOXA9 ectopic expression in U87MG GBM cell line and hTERT/E6/E7 immortalized human astrocytes, and HOXA9 silencing in U251 and GBML18 GBM cell lines. U87MG and hTERT/E6/E7 were retrovirally-infected with an MSCV control vector (MSCV-Control) or with a construct containing the coding region of HOXA9 (MSCV-HOXA9), resulting in U87MG-Control, U87MG-HOXA9, hTERT/E6/E7-Control and hTERT/E6/E7-HOXA9 cell lines. GBML18 and U251 cells were transfected with HOXA9 gene-specific shRNA sequences (shHOXA9) or a non-efective shRNA (shControl) in pGFP-V-RS plasmid, resulting in U251-shControl, U251-shHOXA9, GBML18-shControl and GBML18-shHOXA9 cell lines. Four experimental replicates for HOXA9 overexpression cell lines, and three for HOXA9 silencing cell lines were performed.

2015-01-30 | E-GEOD-56517 | biostudies-arrayexpress

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