Project description:Besides being a common weed, the presence of Echium plantagineum L. in food and feed commodities can represent a safety hazard due to their content in pyrrolizidine alkaloids. In this study, the complete chloroplast of E. plantagineum isolate BPTPS251 is described, being the first available plastome from an isolate belonging to the Echium genus. The chloroplast genome is 149,776 bp in length with 37.5% GC content, displaying a quadripartite structure that contains a pair of inverted repeats regions (25,754 bp each), separated by a large single-copy (80,978 bp) and a small single-copy (17,290 bp) regions. A total of 131 genes were predicted, including 37 tRNA genes, 8 rRNA genes, and 86 protein-coding genes. The phylogenetic analysis confirmed the placement of E. plantagineum under the Boraginaceae family, belonging to the Boraginales order. This study will contribute to conservation, phylogenetic, and evolutionary studies, as well as DNA barcoding applications for food and feed safety purposes.
Project description:The resurrection plant Craterostigma plantagineum possesses an extraordinary capacity to survive long-term desiccation. To enhance our understanding of this phenomenon, complementary transcriptome, soluble proteome and primary metabolome analyses were carried out on plant leaves collected at different physiological stages during a dehydration and rehydration cycle. Dynamic changes in transcript, protein and metabolite levels revealed a unique signature characterizing each stage
Project description:GmMYB176, an R1 MYB transcription factor regulates isoflavonoid biosynthesis in soybean. In the current experiment, GmMYB176 was silenced (GmMYB176-Si) or overexpressed (GmMYB176-OE) in soybean hairy roots and their effect on transcriptome was studied. RNA-Seq analyses of GmMYB176-Si and GmMYB176-OE along with control non-transformed soybean hairy roots revealed that alteration of gene expression of GmMYB176 affects gene regulation of hundreds of genes in soybean.
Project description:We cloned and characterized a subgroup S4 R2R3 Myb transcription factor, PgMyb308-like. Overexpressing PgMyb308-like in pomegranate hairy roots. Transcriptome analysis of PgMyb308-like-overexpressing hairy roots revealed reprogramming of cell wall-related genes, while overexpression of PgMyb308-like in Arabidopsis thaliana plants uncovered its distinct role in a different genetic and metabolic background. The results suggest that PgMyb308-like activates genes in the shikimate pathway and lignin biosynthesis, but suppresses those involved in the production of HTs and flavonoids.
