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Specialized cell state and spatial compartments within the germinal center dark zone

ABSTRACT: Specialized cell state and spatial compartments within the germinal center dark zone

PROVIDER: PRJNA552366 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9637100_1.fastq.gz	Fastqsanger.gz
	SRR9637100_2.fastq.gz	Fastqsanger.gz
	SRR9637110.fastq.gz	Fastqsanger.gz
	SRR9637120.fastq.gz	Fastqsanger.gz
	SRR9637101_1.fastq.gz	Fastqsanger.gz

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Specialized cell state and spatial compartments within the germinal center dark zone

Project description:Within germinal centers (GCs), complex and highly orchestrated molecular programs must balance proliferation, somatic hypermutation (SHM), class switch recombination (CSR) and selection to both provide effective humoral immunity and to protect against genomic instability and neoplastic transformation. In contrast to this complexity, GC B cells are canonically divided into two principal populations, dark zone (DZ) and light zone (LZ) cells. We now demonstrate that following selection in the LZ, B cells migrate to microniches within the canonical DZ that are sites of ongoing cell division. These proliferating DZ (DZp) cells then transit into the larger DZ to become differentiating DZ (DZd) cells before re-entering the LZ. Multidimensional analysis revealed distinct molecular programs in each population commensurate with observed compartmentization of non-compatable functions. These data provide a new three cell zone model that both orders critical GC functions and reveals essential molecular programs of humoral adaptive immunity.

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Project description:Microarrays of gene expression in mouse germinal center B cells photoactivated in the light zone or dark zone, and of naïve cells for comparison. We used microarray data to identify genes differentially expressed by B cells in the light and dark zones of the germinal center. Light and dark zone cells were photoactivated in day 7 germinal centers and sorted by flow cytometry. Naïve splenic B cells(IgD+CD19+) were sorted for comparison.

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Project description:Microarrays of gene expression in human germinal center light zone and dark zone B cells sorted according to the expression of cell surface molecules CD83 and CXCR4 We used microarray data to identify genes differentially expressed by B cells in the light and dark zones of germinal centers from human tonsil specimens. Germinal center B cells (CD19+CD38+IgD-) were sorted from pediatric tonsil discard specimens into light zone (CXCR4hi/CD83lo) and dark zone (CXCR4lo/CD83hi) populations, from which RNA was extracted

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2012-06-13 | E-GEOD-38696 | biostudies-arrayexpress

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2012-06-14 | GSE38697 | GEO

Gene expression in mouse germinal center light zone and dark zone B cells

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