Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells

ABSTRACT: Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells

PROVIDER: PRJNA558254 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR9888152_1.fastq.gz	Fastqsanger.gz
	SRR9888152_2.fastq.gz	Fastqsanger.gz
	SRR9888153_1.fastq.gz	Fastqsanger.gz
	SRR9888153_2.fastq.gz	Fastqsanger.gz
	SRR9888154_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells

Project description:A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether blastomere-like extended pluripotent stem cells (EPS cells) retain a similar generative capacity remains unknown. Here, we established a 3D differentiation system that enabled the generation of blastocyst-like structures from EPS cells (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation. EPS-blastoids formation recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof-of-concept that EPS-blastoids can be generated from somatic cells via cellular reprograming. EPS-blastoids provide a unique platform for studying early embryogenesis, and pave the way to generate viable synthetic embryos using cultured cells.

2019-08-03 | GSE135289 | GEO

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells (single-cell RNA-Seq)

2019-10-17 | GSE135701 | GEO

Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells (single-cell RNA-Seq)

Project description:Generation of Blastocyst-like Structures from Extended Pluripotent Stem Cells (single-cell RNA-Seq)

| PRJNA559790 | ENA

Generation of human blastocyst-like structures from pluripotent stem cells

Project description:Generation of human blastocyst-like structures from pluripotent stem cells

| PRJEB44768 | ENA

Generation of human blastocyst-like structures from pluripotent stem cells

Project description:Generation of human blastocyst-like structures from pluripotent stem cells

| PRJNA667174 | ENA

Generation of human blastocyst-like structures from pluripotent stem cells

Project description:Generation of human blastocyst-like structures from pluripotent stem cells

| PRJNA727994 | ENA

Generation of blastocyst-like structures from human embryonic stem cells I [scRNA-seq]

Project description:We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.

2021-01-24 | GSE150570 | GEO

Generation of blastocyst-like structures from human embryonic stem cells III [scRNA-seq]

Project description:We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.

2021-01-24 | GSE163445 | GEO

Generation of blastocyst-like structures from human embryonic stem cells II [scRNA-seq]

Project description:We established a method that enables the generation of blastocyst-like structures from human pluripotent stem cells.

2021-01-24 | GSE163423 | GEO

Generation of human blastocyst-like structures from pluripotent stem cells

Project description:Due to ethical concerns and restricted access to human blastocysts, we lack a comprehensive understanding of early human embryogenesis. A reliable model system that can recapitulate early stages of human embryogenesis would help solve this problem.Here we report a robust three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids also exhibited the developmental potential to undergo post-implantation morphogenesis in vitro to form structures with a cellular composition and transcriptome signature similar to human embryos that had been cultured in vitro for 8 or 10 days. In conclusion, human EPS-blastoids provide a robust new experimental platform for studying early developmental stages of the human embryo.

2021-09-08 | GSE158971 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data