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Akap11 is a positive regulator of osteogenesis in mouse osteoblastic cell line MC3T3-E1

ABSTRACT: Akap11 is a positive regulator of osteogenesis in mouse osteoblastic cell line MC3T3-E1

PROVIDER: PRJNA562822 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10043130.fastq.gz	Fastqsanger.gz
	SRR10043131.fastq.gz	Fastqsanger.gz
	SRR10043132.fastq.gz	Fastqsanger.gz
	SRR10043133.fastq.gz	Fastqsanger.gz
	SRR10043134.fastq.gz	Fastqsanger.gz

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Akap11 is a positive regulator of osteogenesis in mouse osteoblastic cell line MC3T3-E1

Project description:Purpose: This is a post-GWAS functional study that aims to determine the effect of Akap11 knockout in mouse preosteoblastic cell line MC3T3-E1 during osteogenesis. Methods: mRNA profile of WT (treated with CRISPR and remains unedited) and Akap11 knockout MC3T3-E1 cells were generated by RNAseq from pooled RNA (from 3 biological triplicate at equal quantity) of various timepoint during osteogenesis (Day 0, 7,16, 25).100ng total RNA was used for library construction by KAPA Stranded mRNA-Seq Kit (Roche). Pair-End 101bp sequencing was performed in the Illumin HiSq1500 sequencer using the HiSeq SBS Kit v4 (Illumina). Data was collected with SCS version 1.4.8. Base calling was performed using Illumina’s RTA (Version 1.12.4.2). 7 samples were sequenced per lane and achieved a sequencing depth of ~65 million read per sample. Results: Expression of osteogentic markers in Akap11 knockout MC3T3-E1 cells were suppressed. Genes invovled in IGF-I signaling pathway were signficantly altered.

2019-08-30 | GSE136591 | GEO

Effects of Hsd11b1 overexpressing in mc3T3-e1 pre-osteoblastic cell lines under glucocorticoid excess

Project description:To explore the mechanism by which osteoblastic 11β-HSD1 regulates bone formation and glucose handling, we transfected the mouse MC3T3-E1 osteoblastic cells with a plasmid carrying the exogenous Hsd11b1 or EGFP gene to construct the 11β-HSD1-overexpressed and control osteoblastic cells (hereafter MC3T3-HSD1 and MC3T3-GFP cells), respectively. After incubating with 11-DHC,we then employed RNA sequencing (RNA-seq) to examine the gene expression profile between MC3T3-HSD1 and MC3T3-GFP cells.

2024-09-25 | GSE274790 | GEO

Effects of Hsd11b1 overexpressing in mc3T3-e1 pre-osteoblastic cell lines under glucocorticoid excess (ATAC-Seq)

Project description:To explore the mechanism by which osteoblastic 11β-HSD1 regulates bone formation and glucose handling, we transfected the mouse MC3T3-E1 osteoblastic cells with a plasmid carrying the exogenous Hsd11b1 or EGFP gene to construct the 11β-HSD1-overexpressed and control osteoblastic cells (hereafter MC3T3-HSD1 and MC3T3-GFP cells), respectively. After incubating with 11-DHC,we then employed assay for transposase-accessible chromatin with sequencing (ATAC-seq) to examine the gene expression profile between MC3T3-HSD1 and MC3T3-GFP cells.

2024-09-25 | GSE274974 | GEO

Caspase-9 is a positive regulator of osteoblastic cell migration identified by diaPASEF proteomics

Project description:Caspase-9 is traditionally considered as the initiator caspase of the intrinsic apoptotic pathway. In the last decade, however, other functions beyond initiation/execution of cell death have been described including cell type-dependent regulation of proliferation, differentiation/maturation, mitochondrial and endosomal/lysosomal homeostasis. As previous studies revealed non-apoptotic functions of caspases in osteogenesis and bone homeostasis, this study was performed to identify proteins and pathways deregulated by knockout of caspase-9 in mouse MC3T3-E1 osteoblasts. Data-independent acquisition – parallel accumulation serial fragmentation (diaPASEF) proteomics was used to compare protein profiles of control and caspase-9 knockout cells. A total of 7669 protein groups were quantified and 283 upregulated/141 downregulated protein groups were associated with the caspase-9 knockout phenotype. The deregulated proteins were mainly enriched for those associated with cell migration/motility and DNA replication/repair. Altered migration was confirmed in MC3T3-E1 cells with genetic and pharmacological inhibition of caspase-9. ABHD2, an established regulator of cell migration, was identified as a possible substrate of caspase-9. We conclude that caspase-9 acts as a modulator of osteoblastic MC3T3-E1 cell migration and therefore may be involved in bone remodeling and fracture repair.

2024-04-17 | PXD045703 | Pride

Search for PTH-induced genes in MC3T3-E1 cells

Project description:To identify the changes of mRNA expressions by PTH-treatment for 1 hr in mouse osteoblastic cell line MC3T3-E1, we performed RNA-sequencing (RNA-seq).

2024-06-21 | GSE231552 | GEO

Comparison of gene expression profiles between MC3T3-E1 without osteoblastic induction and with 14 days of ostoblastic induction

Project description:Microarray study revealed 1324 genes that were up-regulated and 1550 genes that were down-regulated more than 1.5 fold change (corrected p<0.05) in MC3T3-E1-derived-mature Osteoblasts compared to control MC3T3-E1.

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Genomic characterization of osteoblastic differentiation of MC-3T3E1 cells

Project description:Gene expression analysis of murine MC3T3-E1 cells induced to undergo synchronized osteoblastic differentiation in vitro.

2012-06-28 | GSE30393 | GEO

Expression Data from MC3T3-E1 cells subjected to laminar fluid flow (LFF)

Project description:Osteoblasts are responsive to shear stress. We investigated the effect of of laminar fluid flow (LFF) on osteoblast-like MC3T3-E1 cells at two timepoints. We used microarray analysis to detail the global gene expression of MC3T3-E1 cells in response to 1 hour of laminar fluid flow directly and 4 hours after treatment.

2021-02-15 | GSE138160 | GEO

Identification of differentially expressed genes associated with dimethyl sulfoxide-enhanced mineralization in mouse MC3T3-E1 preosteoblast cells.

Project description:Dimethyl sulfoxide (DMSO) is widely used as a solvent for water-insoluble substances and has biological properties such as inducing cellular differentiation, scavenging free radicals, and acting as a radioprotectant. DMSO has also been reported to enhance mineralization in osteoblastic cells. However, its molecular mechanisms have not yet been fully elucidated. In this study, we identified the differentially expressed genes associated with dimethyl sulfoxide-enhanced mineralization in mouse MC3T3-E1 preosteoblast cells using GeneChip® oligonucleotide microarrays.

2024-08-12 | GSE274013 | GEO

mice mRNA array for MACF1-knockdown MC3T3-E1 cell line

Project description:MC3T3-E1 cells with stable knockdown of MACF1 by shRNA. Cells were normally cultured and RNA extracted for mRNA microarray.

2022-02-02 | E-MTAB-11425 | biostudies-arrayexpress