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Plant toxin treatment of neonatal mouse cholangiocytes

ABSTRACT: Plant toxin treatment of neonatal mouse cholangiocytes

PROVIDER: PRJNA564001 | ENA |

REPOSITORIES: ENA

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Plant toxin treatment of neonatal mouse cholangiocytes

Project description:Extrahepatic bile ducts were isolated from mouse pups at days 0-3 and primary cholangiocytes were isolated. Cholangiocytes were treated with DMSO, bilatresone (TOX4), betavulgarin (TOX2), and isoflavanone (TOX3), as per Lorent et al, Science Translationa Medicine 2015;286:286ra67 (Fig. 1), all in DMSO. Treatment concentrations were 2.0 micrograms/ml, for 6 hours.

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Project description:Transcriptional profiling of log and stationary phases of S. Typhimurium, comparing untreated controls with T2 toxin-treated samples. Each array used labelled cDNA against a common genomic DNA reference. Trplicate arrays were carried out for each of the 4 conditions: untreated log phase, untreated stationary phase, T2 toxin treated log phase and T2 toxin treated stationary phase.

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