Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell Sequencing of adipose Treg cells

ABSTRACT: Single-cell Sequencing of adipose Treg cells

PROVIDER: PRJNA565725 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10123975_1.fastq.gz	Fastqsanger.gz
	SRR10123975_2.fastq.gz	Fastqsanger.gz
	SRR10123976_1.fastq.gz	Fastqsanger.gz
	SRR10123976_2.fastq.gz	Fastqsanger.gz
	SRR10123977_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

Single-cell Sequencing of adipose Treg cells

Project description:Systematic characterizations of adipose Treg subsets and their in vivo roles remain uncommon. Using paired single-cell RNA-seq and TCR-seq to map adipose Treg cells, we identified conserved adipose subsets with distinct clonal expansion patterns. By gain and loss of function studies, we demonstrated adipose Treg subsets display disparate influences. To understand the clonal structure of adipose Treg cells, we analyzed their TCR sharing data and observed a number of overlapped TCR clonotypes, suggesting a cellular state transition between adipose Treg clusters. Our findings have important implications for understanding phenotypic diversity of tissue Tregs in vivo.

2021-07-15 | GSE137493 | GEO

Single-cell ATAC-Sequencing of adipose Treg cells

Project description:Single-cell ATAC-Sequencing of adipose Treg cells

| PRJNA616335 | ENA

Single-cell ATAC-Sequencing of adipose Treg cells

Project description:Systematic characterizations of adipose Treg subsets and their in vivo roles remain uncommon. The goal of this study is to reveal the heterogeneity and chromatin landscape of adipose Treg cells. Using single-cell ATAC-seq to map adipose Treg cells, we identified different adipose subsets. Strikingly, our scATAC-seq analysis revealed a strong enrichment of key transcription factor motifs, which may play function in fate specification. By gain and loss of function studies, we further demonstrated adipose Treg subsets display disparate physiologic influences. Our findings have important implications for understanding phenotypic diversity of tissue Tregs in vivo.

2021-07-15 | GSE147768 | GEO

Single-cell RNA sequencing data (SORT-seq) of Treg cells in the human postnatal thymus

Project description:We have performed single-cell RNA sequencing to investigate human regulatory T cell development in the thymus. Treg cells were isolated from a postnatal thymus derived from a 7-week old infant. We examined the composition of the Treg cell compartment in the human thymus and aimed to dissect developmental pathways in Treg cell development.

2021-11-08 | E-MTAB-11019 | biostudies-arrayexpress

Single-cell RNA-seq of murine thymic Treg cell progenitors and mature Treg cells

Project description:Single-cell RNA-seq of murine thymic Treg cell progenitors and mature Treg cells

| PRJNA507435 | ENA

Single-cell sequencing of adipose-derived mesenchymal stromal cells and dermal fibroblasts

Project description:Single-cell sequencing of adipose-derived mesenchymal stromal cells and fibroblasts.

| EGAS00001006954 | EGA

Next Generation Sequencing Facilitates Quantitative Analysis of adipose tissue Treg and LN Treg Transcriptomes

Project description:The goal of this study is to reveal unique features of adipose Tregs and to study mechanisms underlying how mediator Med23 regulate adipose Treg function in aged mice. Therefore, mRNA profiles of LN Tregs (from 4-month old Foxp3Cre mice) and adipose Tregs (from 4-month old Foxp3Cre, 4-month old obese Foxp3Cre, 10-month old Foxp3Cre and 10-month old Med23fl/fl;Foxp3Cre mice) were generated by deep sequencing, single experiment, using Illumina HiSeq2000. We compared transcriptome features between lymph node-derived Tregs and adipose Tregs from 4-month old lean or obese mice. Using unbiased comparative gene expression analyses, we found adipose Tregs display an up-signature of Insr (Insulin receptor) and Hif1a, while Pparg acts as a positive control. We next compared gene expression profiles of adipose Tregs from 10-month old Med23fl/fl;Foxp3Cre (MKO) and Foxp3Cre (WT) mice. adipose Med23-ΔTreg cells display impaired transcription of Pparg and Il1rl1 (ST2), and they simultaneously acquire the expression of Nt5e (CD73), while Entpd1 (CD39) expression was not dramatically altered. Our studies implicate protective roles of CD73hi adipose Tregs and offer new therapeutic strategies against age-associated metabolic syndrome.

2021-07-15 | GSE124721 | GEO

Single-cell RNA-seq of murine thymic Treg cell progenitors and mature Treg cells

Project description:We use single-cell RNA-seq to determine distinct selection phenotypes of 2 rare thymic Treg cell progenitors as well as mature thymic Treg cells

2018-12-31 | GSE123067 | GEO

Single-cell RNA sequencing Analysis of Treg cells from mouse B16 melanoma tumors

Project description:Based on single-cell RNA sequencing technology, we studies gene expression profiles in Treg cells isolated from mouse tumors before and after rMVA therapy. We identified differential Treg populations from tumors before and after rMVA therapy. The difference of Treg clusters in tumors were also identified.

2023-03-23 | GSE199371 | GEO

Single cell RNA-sequencing of visceral adipose tissue Pdgfrβ+ cells

Project description:Single cell RNA-sequencing of visceral adipose tissue Pdgfrβ+ cells

| PRJNA437472 | ENA