Project description:We report the transcriptional response to Colorado potato beetle herbivory in leaves of the highly beetle resistant Solanum chacoense diploid line USDA8380-1 (80-) and a susceptible F2 individual (EE501F2_093) derived from a cross between 80-1 and a beetle susceptible line S. chacoense M6. Sampling tissue in a time course during adult Colorado potato beetle feeding provides novel insight to the transcriptomic defense response to this important pest.
Project description:In the present study molecular interactions between potato plants, Colorado potato beetle (CPB) larvae and Potato virus YNTN (PVYNTN) were investigated by analyzing gene expression in potato leaves. Grant ID: J4-4165 Slovenian Research Agency ARRS Growth and defense trade-offs in multitrophic interaction between potato and its two major pests Grant ID: P4-0165 Slovenian Research Agency ARRS Biotechnology and Plant Systems Biology
Project description:In the present study molecular interactions between potato plants, Colorado potato beetle (CPB) larvae and Potato virus YNTN (PVYNTN) were investigated by analyzing gene expression in potato leaves. mRNA samples of secondary PVYNTN-infected (CPB_PVY) and healthy potato plants (CPB_H) cultivar Igor and of RNAi coi1-silenced (CPB_coi1) and non-transformed (CPB_NT) potato plants cultivar Desiree collected 24 h post CPB infestation and respective control non-infested samples (CONT_PVY, CONT_H, CONT_coi1, CONT_NT).
Project description:Protein complexes of aegerolysins pleurotolysin A2 (PlyA2) and pleurotolysin B (PlyB) from oyster mushrooms Pleurotus sp. display targeted toxicity against Colorado potato beetle (CPB; Leptinotarsa decemlineata) larvae. This selective toxicity is achieved through aegerolysins' interaction with insect-specific membrane sphingolipids. This study explores the potential adaptive response of CPB larvae to the aegerolysin complex.
Project description:Glycoalkaloids are Solanum bioactive compounds and are involved in allelopathic interactions. To achieve a better understanding of plant–plant interactions, it is essential to deeply examine the trait distribution in a segregating population. In the present paper, we used transcriptomic and metabolomic approaches to recognize the phytotoxic abilities of potato plants originating from a diploid segregating F1 population with particular emphasis on glycoalkaloids in potato groups characterized by a high glycoalkaloid content. In potato F1 individuals, six glycoalkaloids were recognized: solasonine, solamargine, α-solanine, α-chaconine, leptinine I, and leptine II. In the bulk samples characterized by a high total glycoalkaloid content and various phytotoxic potential, high - A’, low - B’ and hormesis - F’, a significant role of the glycoalkaloid composition in the expression of phytotoxic potential was revealed. In particular, leptine II, solasonine and solamargine were present. Based on the RNA-seq analysis of the bulk samples, a flavonol synthase/flavanone 3-hydroxylase-like gene responsible for flavonoid synthesis was upregulated in comparison A’ vs. B’ and A’ vs. F’ (Log2FC=8.30 and 6.40, respectively). The population-level evaluation of phytotoxic potential confirmed a significant negative correlation between total glycoalkaloid content and phytotoxic potential (r=-0.211) but only after correction for total flavonoid content.
2021-10-27 | GSE186482 | GEO
Project description:Metagenomics of Colorado Potato Beetle
