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Circulating traveling waves rapidly pace and mature hiPSC-derived cardiomyocytes in self-organized tissue ring

ABSTRACT: Circulating traveling waves rapidly pace and mature hiPSC-derived cardiomyocytes in self-organized tissue ring

PROVIDER: PRJNA589853 | ENA |

REPOSITORIES: ENA

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Circulating traveling waves rapidly pace and mature hiPSC-derived cardiomyocytes in self-organized tissue ring

Project description:Directed differentiation methods allow acquisition of high-purity cardiomyocytes (CMs) differentiated from human induced pluripotent stem cells (hiPSCs); however, their immaturity characteristic limits their application for drug screening and regenerative therapy. The rapid electrical pacing of cardiomyocytes have been used for efficiently promoting the maturation of cardiomyocytes, here we describe a simple device in modified culture plate on which hiPSC-derived CMs can form three-dimensional self-organized tissue rings (SOTRs). Using calcium imaging, we show that within the ring, traveling waves (TWs) of action potential spontaneously originated and ran robustly at a frequency up to 4 Hz. After 2 weeks, SOTRs with TW training show matured features including structural organization, increased cardiac-specific gene expression, enhanced Ca2+-handling properties, an increased oxygen-consumption rate, and enhanced contractile force. We subsequently use a mathematical model to interpret the origination, propagation, and long-term behavior of the TWs within the SOTRs. The TW could also potentially be used for pacing the electrical excitable cells such as neuron and retina cells for various applications.

2020-02-05 | GSE140466 | GEO

Circulating re-entrant waves promote maturation of hiPSC-derived cardiomyocytes in self-organized tissue ring.

Project description:Directed differentiation methods allow acquisition of high-purity cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs); however, their immaturity characteristic limits their application for drug screening and regenerative therapy. The rapid electrical pacing of cardiomyocytes has been used for efficiently promoting the maturation of cardiomyocytes, here we describe a simple device in modified culture plate on which hiPSC-derived cardiomyocytes can form three-dimensional self-organized tissue rings (SOTRs). Using calcium imaging, we show that within the ring, reentrant waves (ReWs) of action potential spontaneously originated and ran robustly at a frequency up to 4 Hz. After 2 weeks, SOTRs with ReWs show higher maturation including structural organization, increased cardiac-specific gene expression, enhanced Ca2+-handling properties, an increased oxygen-consumption rate, and enhanced contractile force. We subsequently use a mathematical model to interpret the origination, propagation, and long-term behavior of the ReWs within the SOTRs.

| S-EPMC7070090 | biostudies-literature

Transcription profiling of Plasmodium falciparum celllines, CD36 panned clones (Ring) hybridized against S8.4 (Ring)

Project description:Plasmodium falciparum cellline experiment: CD36 panned clones (Ring) were hybridized against S8.4 (Ring)

2007-04-25 | E-MEXP-439 | biostudies-arrayexpress

Ring Trial Food

Project description:Ring Trial COMPARE project

| PRJEB28170 | ENA

COMPARE FFPE Ring trial

Project description:COMPARE FFPE Ring trial

| PRJEB32129 | ENA

Self-organized emergence of hyaline cartilage in hiPSC-derived multi-tissue organoids

Project description:Here, we developed a simple xeno- and feeder-free protocol for human hyaline cartilage production in vitro using hydrogel-cultured multi-tissue organoids (MTO). We investigate gene regulatory networks during spontaneous hiPSC-MTO differentiation using RNA sequencing and bioinformatic analyses. We find the interplays between BMPs and neural FGF pathways are associated with MTO phenotype transition. We recognize TGF-beta/BMP and Wnt signaling likely contribute to the long-term maintenance of cartilage growth and specific increase in articular cartilage development. By comparing MTO cartilage transcription signature with human lower limb chondrocytes, we observe MTO chondrocytes show strong correlation with fetal lower limb cartilage tissues. Collectively, our findings describe self-organized emergence of MTO hyaline cartilage, its associated molecular pathways, and spontaneous adoption of articular cartilage development trajectories by MTO.

2022-09-12 | GSE184007 | GEO

Metagenomic Ring Trial

Project description:COMPARE Food Metagenomic Ring Trial 2018 - spiked salmon

| PRJEB27267 | ENA

COMPARE food metagenomic ring trial

Project description:COMPARE food metagenomic ring trial

| PRJEB28753 | ENA

PiGutNet Ring Test

Project description:Ring Test for fecal DNA extraction protocols in pigs

| PRJEB24564 | ENA

Genome-wide transcriptional analysis of Drosophila ring gland

Project description:Samples 1-24: Tissue-specific gene expression microarrays (Nimblegen) using dissected ring glands isolated from FOUR different time points of control (w1118, otherwise wild type) third instar larvae. Time points are 4, 8, 24 and 36 hours after the molt from second to third instar larvae. Samples 25-42: Tissue-specific gene expression microarrays (Nimblegen) using dissected ring glands isolated from TWO different time points of third instar larvae. Genotypes were phantom22-GAL4/RasV12 and phantom22/Torso-RNAi. Goal was to identify PTTH-dependent gene sets in the ring gland. Time points were 18 hours and 8 hours prior to puparium formation.

2016-06-20 | GSE80475 | GEO

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