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E2A Specifies Adaptive Immunity by Instructing Large-Scale Topological Changes

ABSTRACT: E2A Specifies Adaptive Immunity by Instructing Large-Scale Topological Changes

PROVIDER: PRJNA592719 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10566600.fastq.gz	Fastqsanger.gz
	SRR10566601.fastq.gz	Fastqsanger.gz
	SRR10566602.fastq.gz	Fastqsanger.gz
	SRR10566603.fastq.gz	Fastqsanger.gz
	SRR10566604.fastq.gz	Fastqsanger.gz

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E2A Specifies Adaptive Immunity by Instructing Large-Scale Topological Changes

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Project description:Background: E2A, encoded by the TCF3 gene locus, belongs to the E protein transcription factor family, which also includes HEB (TCF12) and E2-2 (TCF4), has been suggested to play an important role in leukemogenesis. However, far less is known about the function of E2A in cell-fate regulation of hESCs. Therefore, further understanding of E2A in self-renewal and differentiation of embryonic stem cells may be influenced. Methods: The mRNA profiles of wildtype and E2A knockout embryonic stem cells were generated by RNA-seq technique, in triplicate for each group, using IIIumina Hiseq 2500. Results: A comprehensive human transcriptome map of wild type and E2A knockout embryonic stem cells was provided. Function enrichment, network characteristics and disease association of the differentially expressed genes were analyzed. Conclusion: The dataset could serve as a baseline resource for investigating the potential effects and mechanism of E2A in embryonic stem cells.

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Gene expression profiling of the Se-Ax cell line following E2A reconstitution

Project description:The transcription factor E2A is essential for lymphocyte development. In this study, we describe a recurrent E2A gene deletion in at least 70% of patients with Sézary syndrome (SS), a subtype of T cell lymphoma. Loss of E2A results in enhanced proliferation and cell cycle progression via derepression of the proto-oncogene MYC and the cell cycle regulator CDK6. Furthermore, by examining the gene expression profile of SS cells following restoration of E2A expression, we identify a number of E2A-regulated genes that interfere with oncogenic signaling pathways including the Ras pathway. Several of these genes are down-regulated or lost in primary SS tumor cells. These data demonstrate a tumor suppressor function of E2A in human lymphoid cells and could help to develop new treatment strategies for human lymphomas with altered E2A activity.

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HEB and E2A function as SMAD/FOXH1 cofactors

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