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Nicotiana benthamiana cultivar:Tobacco Bright Yellow

ABSTRACT: Nicotiana benthamiana cultivar:Tobacco Bright Yellow Raw sequence reads

PROVIDER: PRJNA600298 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13528179_1.fastq.gz	Fastqsanger.gz
	SRR13528179_2.fastq.gz	Fastqsanger.gz
	SRR13528180_1.fastq.gz	Fastqsanger.gz
	SRR13528180_2.fastq.gz	Fastqsanger.gz

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Strigolactones negatively regulate Tobacco mosaic virus resistance in Nicotiana benthamiana

Project description:Strigolactones (SLs) are plant hormones that regulate diverse developmental processes and environmental responses in plants. It has been discovered that SLs play an important role in regulating plant immune resistance to pathogens, but there are currently no reports on their role in the interaction between Nicotiana benthamiana and Tobacco mosaic virus (TMV). In this study, the exogenous application of SLs weakened the resistance of N. benthamiana to TMV, promoting TMV infection, whereas the exogenous application of Tis108, an SL inhibitor, resulted in the opposite effect. Virus-induced gene silencing (VIGS) inhibition of two key SL synthesis enzyme genes, NtCCD7 and NtCCD8, enhanced the resistance of N. benthamiana to TMV. Additionally, we conducted a screening of N. benthamiana related to TMV infection. TMV-infected plants treated with SLs were compared to the control by using RNA-seq. KEGG enrichment analysis and weighted gene co-expression network analysis (WGCNA) of differentially expressed genes (DEGs) suggested that plant hormone signaling transduction may play a significant role in the SL-TMV-N. benthamiana interactions. This study reveals new functions of SLs in regulating plant immunity and provides a reference for controlling TMV diseases in production.

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