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Genome-wide investigation of the dynamic changes of epigenome modifications after DNA methylation editing

ABSTRACT: Genome-wide investigation of the dynamic changes of epigenome modifications after DNA methylation editing

PROVIDER: PRJNA605243 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10972409.fastq.gz	Fastqsanger.gz
	SRR10972410.fastq.gz	Fastqsanger.gz
	SRR10972411.fastq.gz	Fastqsanger.gz
	SRR10972412.fastq.gz	Fastqsanger.gz
	SRR10972413.fastq.gz	Fastqsanger.gz

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Project description:Chromatin properties are regulated by complex networks of epigenome modifications. Currently, it is unclear how these modifications interact and if they control downstream effects such as gene expression. We employed promiscuous chromatin binding of a zinc finger fused catalytic domain of DNMT3A to introduce DNA methylation in HEK293 cells at many CpG islands (CGIs) and systematically investigated the dynamics of the introduced DNA methylation and the consequent changes of the epigenome network. We observed efficient methylation at thousands of CGIs, but it was unstable at about 90% of them, highlighting the power of genome-wide molecular processes that protect CGIs against DNA methylation. Partially stable methylation was observed at about 1000 CGIs, which showed enrichment in H3K27me3. Globally, the introduced DNA methylation strongly correlated with a decrease in gene expression indicating a direct effect. Similarly, global but transient reductions in H3K4me3 and H3K27ac were observed after DNA methylation but no changes were found for H3K9me3 and H3K36me3. Our data provide a global and time-resolved view on the network of epigenome modifications, their connections with DNA methylation and the responses triggered by artificial DNA methylation revealing a direct repressive effect of DNA methylation in CGIs on H3K4me3, histone acetylation, and gene expression.

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Genome-wide investigation of the dynamic changes of epigenome modifications after DNA methylation editing

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-11-10 | GSE144904 | GEO

Investigation of RNA editing sites within bound regions of RNA-binding proteins

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