Project description:Mycobacterium tuberculosis is the causitive agent of TB, which is a global epidemic that has claimed millions of lives. Some clinical strains of M.tb have significantly more severe clinical outcomes, while some other mycobacteria ordinarily do not cause disease in humans. The biological processes underpinning this difference are poorly understood. Thus, this project aimed to identify proteomic differences between more virulent strains of the MTBC (Beijing, J37Rv, CAS and LAM) vs those strains that only cause disease in severely immune compromised humans (Bovis, Smegmatis and Avium). We hypothesise that these differences may offer insight into the molecular mechanisms by which M.tb has become more virulent by evading drug treatment or evading the host immune system more effectively.
Project description:Tuberculosis (2004) 84, 188196 (www.elsevierhealth.com/journals/tube),Comparative expression studies of a complex phenotype: cord formation in Mycobacterium tuberculosis,Qian Gao, Katharine Kripke, Zuhre Arinc, Martin Voskuil, Peter Small
Project description:Mycobacterium tuberculosis, the causative agent of tuberculosis, is one of the leading causes of death due to infectious disease. The Mammalian Cell Entry proteins are essential virulence factors during infection and are thought to facilitate the import of nutrients across the highly complex mycobacterial cell envelope. We purified an endogenous Mammalian Cell Entry complex from the model mycobacterium, Mycobacterium smegmatis, identified components of the Mce1 protein complex using mass spectrometry, and determined its structure using single-particle cryo-electron microscopy and AlphaFold2. This entry contains the mass spectrometry raw files and search results for the LC-MS analysis of the purified complex.
