Ontology highlight
ABSTRACT:
PROVIDER: PRJNA623014 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Wadden Sea marine benthic metabarcoding 18S
| PRJEB33084 | ENA
Project description:Environmental metabarcoding for monitoring benthic communities
| PRJNA369002 | ENA
Project description:Background: Marine phytoplankton are responsible for 50% of the CO2 that is fixed annually worldwide and contribute massively to other biogeochemical cycles in the oceans. Diatoms and coccolithophores play a significant role as the base of the marine food web and they sequester carbon due to their ability to form blooms and to biomineralise. To discover the presence and regulation of short non-coding RNAs (sRNAs) in these two important phytoplankton groups, we sequenced short RNA transcriptomes of two diatom species (Thalassiosira pseudonana, Fragilariopsis cylindrus) and validated them by Northern blots along with the coccolithophore Emiliania huxleyi. Results: Despite an exhaustive search, we did not find canonical miRNAs in diatoms. The most prominent classes of sRNAs in diatoms were repeat-associated sRNAs and tRNA-derived sRNAs. The latter were also present in E. huxleyi. tRNA-derived sRNAs in diatoms were induced under important environmental stress conditions (iron and silicate limitation, oxidative stress, alkaline pH), and they were very abundant especially in the polar diatom F. cylindrus (20.7% of all sRNAs) even under optimal growth conditions. Conclusions: This study provides first experimental evidence for the existence of short non-coding RNAs in marine microalgae. Our data suggest that canonical miRNAs are absent from diatoms. However, the group of tRNA-derived sRNAs seems to be very prominent in diatoms and coccolithophores and may be used for acclimation to environmental conditions.
2015-03-06 | GSE57987 | GEO
Project description:The biomolecular composition of diatom underwater adhesives remains unknown as does the precise mechanism by which the secretion of adhesive material from the raphe slit is coupled to an intracellular motor that provides the force for cell motility. In this study we have characterized the previously identified frustule associated components (FACs) from the common fouling diatom Craspedostauors australis and demonstrated that they form a continuous cell wall coating. The FACs are encoded by a single polypeptide (caFAP1) that has a domain structure of alternating cysteine-rich and PTS-rich regions which is reminiscent of the gel-forming mucins. CaFAP1 is notably absent from the raphe slit and therefore does not play a direct role in cell adhesion and motility but may rather play an important role in lubrication and self-cleaning that is required for epipsammic benthic diatoms that grow attached to sand grains, or moving through sand.
2023-01-09 | PXD026968 | Pride
Project description:Preservative DNA metabarcoding of benthic macroinvertebrates from the Vjosa catchment
| PRJEB51480 | ENA