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Craspedostauros australis frustule associated components analysis

ABSTRACT: The biomolecular composition of diatom underwater adhesives remains unknown as does the precise mechanism by which the secretion of adhesive material from the raphe slit is coupled to an intracellular motor that provides the force for cell motility. In this study we have characterized the previously identified frustule associated components (FACs) from the common fouling diatom Craspedostauors australis and demonstrated that they form a continuous cell wall coating. The FACs are encoded by a single polypeptide (caFAP1) that has a domain structure of alternating cysteine-rich and PTS-rich regions which is reminiscent of the gel-forming mucins. CaFAP1 is notably absent from the raphe slit and therefore does not play a direct role in cell adhesion and motility but may rather play an important role in lubrication and self-cleaning that is required for epipsammic benthic diatoms that grow attached to sand grains, or moving through sand.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Craspedostauros Australis

TISSUE(S): Photosynthetic Cell, Cell Culture

SUBMITTER: Nicole Poulsen

LAB HEAD: Nicole Poulsen

PROVIDER: PXD026968 | Pride | 2023-01-09

REPOSITORIES: Pride

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			Action	DRS
	20190624_SDS_Gels_Nicole_Poulsen.pdf	Pdf
	20190630_NP0101_RTSTD16fm_090min_01.mgf	Mgf
	20190630_NP0101_RTSTD16fm_090min_01.raw	Raw
	20190630_NP0101_RTSTD16fm_090min_01_F006399.dat	Other
	20190630_NP0102_RTSTD16fm_090min_01.mgf	Mgf

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