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Porcine neutrophils infected by Salmonella

ABSTRACT: Porcine neutrophils infected by Salmonella

PROVIDER: PRJNA623565 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR11497600.fastq.gz	Fastqsanger.gz
	SRR11497601.fastq.gz	Fastqsanger.gz
	SRR11497602.fastq.gz	Fastqsanger.gz
	SRR11497603.fastq.gz	Fastqsanger.gz
	SRR11497604.fastq.gz	Fastqsanger.gz

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Project description:The GeneChip Porcine Genome Array was used to identify the transcriptional response upon either Salmonella typhimurium (ST) or Salmonella choleraesuis (SC) infection in two porcine epithelial cell lines (IPEC-J2, from jejunum and IPI-2I, from ileum) during 2 and 4 hours post infection. The objectives in this study were first, to identify the different response between the epithelial cell lines from different gut regions; second, to study how the Salmonella serotypes used could elicit a different host response; and third, to determine the effect of the time-points on the differentially gene expression. Epithelial cells were seeded into 6-well tissue culture plates and grown to confluence in 5% CO2 at 37ÂºC. Monolayers were infected for 1 h. with Salmonella typhimurium or Salmonella choleraesuis serotypes (MOI 1:10) or incubated with media alone (Control cells). Extracellular bacteria were removed, and cultures were further incubated during 2 and 4 h. in the presence of 50 Âµg/ml of the non-cell-permeant antibiotic gentamicin to kill remaining extracellular bacteria. The experiment was developed in triplicate in order to ensure a proper robustness in the microarray statistical analysis. An indirect ELISA was used to confirm cell activation by means of IL8 detection in cell culture medium. After each time-point, cells were lysed and RNA extracted.

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