Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of AKP organoids over different stages of differentiation

ABSTRACT: RNA-seq of AKP organoids over different stages of differentiation

PROVIDER: PRJNA624033 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq of AKP organoids over different stages of differentiation

Project description:Transcriptomic profiling of mouse tumoroids at early (2 days of Apc restoration) versus late stage differentiation (7 days of Apc restoration)

2020-05-22 | GSE148352 | GEO

Transcriptomic data from cultured human fetal lung tip-derived organoids at different stages of lung development

Project description:Human fetal lung tip cells were purifed from developing human lungs at pseudoglandular and canalicular stages and in vitro cultured as organoids. Whole transcriptomic data for each organoid line at different stages were profiled by RNA seq.

2022-09-30 | GSE178528 | GEO

Genome-wide chromatin accessibility data from cultured human fetal lung tip-derived organoids at different stages of lung development

Project description:Human fetal lung tip cells were purifed from developing human lungs at pseudoglandular and canalicular stages and in vitro cultured as organoids. Chromatin accessibility data for each organoid line at different stages were profiled by ATAC-seq.

2022-09-30 | GSE178527 | GEO

LC MS/MS of kidney organoids during differentiation from day 21 to 29

Project description:Kidney organoids are a valuable and innovative model to understand genetic diseases, kidney development and transcriptomic dynamics. However, their proteome has not been analyzed so far. Here, we analyzed the organoid proteome trajectory during differentiation. Genes involved in podocytopathies and cystic kidney diseases were abundantly expressed on protein level, distinguishing organoids from almost every available cell culture model. On their pathway to terminal differentiation, organoids developed increased deposition of extracellular matrix. Single cell transcriptomic analysis suggests that most changes locate to podocytes and early podocyte progenitors. This matrix deposition is different from commonly used animal models of glomerular disease. We grew organoids from two independent batches according to the Freedman protocol, and performed proteomic profiling (Freedman, Brooks et al. 2015, Czerniecki, Cruz et al. 2018). The IPSCs were differentiated for a three-week period until first spheroids from. From day 21 of the culture they were used in our experiments up until day 29, where off-target differentiation of organoids becomes an issue.

2023-05-12 | PXD029716 | Pride

scRNA-seq and snucRNA-seq of human primary trophoblast organoids (PTO) and trophoblast stem cells (TSCs) differentiation into extravillous trophoblast organoids (EVTs)

Project description:This study aims to compare in vivo human trophoblast differentiation into EVTs to different in vitro trophoblast organoids using single-cell and single-nuclei RNA sequencing. The study includes two type of systems: human primary trophoblast organoids (PTO) and trophoblast stem cells (TSCs). Trophoblast stem cell (TSC) lines BTS5 and BTS11 derived by Okae and colleagues were grown as described previously (Okae et al. 2018) and together with EVT media. Primary trophoblast organoids (PTO) were grown and differentiated into EVT as previously described by Turco & Sheridan (Turco et al 2018; Sheridan et al 2020). This study shows that the main regulatory programs mediating EVT invasion in vivo are preserved in in vitro models of EVT differentiation from primary trophoblast organoids and trophoblast stem cells.

2023-02-09 | E-MTAB-12650 | biostudies-arrayexpress

Single-Cell RNA Sequencing of Retinal Organoids Derived by CSC14 hESCs

Project description:Retinal organoids samples that derived from human embryonic stem cells were analyzed by single-cell RNA sequencing. Two samples at different differentiation stages (day57 and day 171) were included in this study for cell type comparison.

2022-01-01 | E-MTAB-11121 | biostudies-arrayexpress

RNA-Seq analysis of human skeletal muscle organoids

Project description:We performed RNA sequencing analyses of human iPSC and iPSC-derived skeletal muscle organoids in different differentiation stages of 4wk, 8wk, and 16wk.

2023-06-09 | GSE147513 | GEO

Comparison of bioprinted kidney organoids in different conformations

Project description:Bulk RNA-seq comparison of kidney organoids bioprinted in 3 different conformations with varying starting cell densities. Density is dictated by the ratio of bioprinter tip movement to the amount of extrusion, where higher ratios spread cells over a larger surface area. We compare organoids printed with no movement ('blob', ratio 0) to those with moderate ('line 3', ratio 20) or high movement ('line 1', ratio 40).

2020-09-01 | GSE138733 | GEO

Comparative analysis of WT and ZEB1 KO cells in different stages during differentiation

Project description:Comparative analysis of WT and ZEB1 KO cells in different stages during differentiation

| PRJNA487626 | ENA

RNA sequencing of iAM-1 cells at different stages during one cycle of differentiation and subsequent dedifferentiation

Project description:RNA sequencing of iAM-1 cells at different stages during one cycle of differentiation and subsequent dedifferentiation

2024-01-13 | GSE164763 | GEO