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URT1-mediated uridylation shapes poly(A) tails in Arabidopsis

ABSTRACT: URT1-mediated uridylation shapes poly(A) tails in Arabidopsis

PROVIDER: PRJNA624145 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11521300_1.fastq.gz	Fastqsanger.gz
	SRR11521300_2.fastq.gz	Fastqsanger.gz
	SRR11521281_1.fastq.gz	Fastqsanger.gz
	SRR11521281_2.fastq.gz	Fastqsanger.gz
	SRR11521282_1.fastq.gz	Fastqsanger.gz

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URT1-mediated uridylation shapes poly(A) tails in Arabidopsis

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Project description:URT1-mediated uridylation shapes poly(A) tails in Arabidopsis

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Project description:3’ uridylation is increasingly recognized as a conserved RNA modification process associated with RNA turnover in eukaryotes. 2’-O-methylation on the 3’ terminal ribose protects micro(mi)RNAs from 3’ truncation and 3’ uridylation in Arabidopsis. Previously, we identified HESO1 as the nucleotidyl transferase that uridylates most unmethylated miRNAs in vivo, but substantial 3’ tailing of miRNAs still remains in heso1 loss-of-function mutants. In this study, we found that among nine HESO1 paralogs, UTP:RNA URIDYLYLTRANSFERASE 1 (URT1) is the single most predominant nucleotidyl transferase that tails miRNAs. URT1 and HESO1 prefer substrates with different 3’ end nucleotides in vitro and act cooperatively to tail different forms of the same miRNAs in vivo. Moreover, both HESO1 and URT1 exhibit nucleotidyl transferase activity on AGO1-bound miRNAs. Although these enzymes are able to add long tails to AGO1-bound miRNAs, the tailed miRNAs remain associated with AGO1. In fact, we show that a tailed miRNA acquires the ability to trigger the production of secondary siRNAs. Therefore, 3’ uridylation could endow new properties to miRNAs in addition to its known effects in miRNA degradation.

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