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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [2D culture]

ABSTRACT: Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [2D culture]

PROVIDER: PRJNA636817 | ENA |

REPOSITORIES: ENA

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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma

Project description:Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma

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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [tfap2a/e]

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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [RNA-Seq]

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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma

Project description:This SuperSeries is composed of the SubSeries listed below.

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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [3D culture]

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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [tfap2a/e]

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Cell state diversity promotes metastasis through heterotypic cluster formation in melanoma [RNA-Seq]

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Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis

Project description:Circulating tumor cell clusters/micro-emboli (CTM) possess greater metastatic capacity and survival advantage compared to individual circulating tumor cell (CTCs). However, the formation of CTM subtypes and their role in tumor metastasis remain unclear. In this study, we used a microfluidic Cluster-chip with easy operation and high efficiency to isolate CTM from peripheral blood, which confirmed their correlation with clinicopathological features and identified the critical role of CTC-platelet clusters in BC metastasis. The correlation between platelets and CTM function was further confirmed in a mouse model and RNA-seq of CTM identified high-expressed genes related to hypoxia stimulation and platelet activation which possibly suggested the correlation of hypoxia and CTC-platelet cluster formation. In conclusion, we successfully developed the Cluster-chip platform to realize the clinical capture of CTMs and analyze the biological properties of CTC-platelet clusters, which could benefit the design of potential treatment regimens to prevent CTM-mediated metastasis and tumor malignant progression.

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