Ontology highlight
ABSTRACT:
PROVIDER: PRJNA645038 | ENA |
REPOSITORIES: ENA
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Project description:The objective of the proposed pilot study is to identify metabolites up- and down-regulated in African bermudagrass that are tolerant and sensitive to the sting nematode and develop metabolomic markers for the highest expressed metabolites associated with tolerance. Future work will include additional accessions and species of bermudagrass, and testing under field conditions. Bermudagrass accessions identified as tolerant or sensitive by Pang et al. (2011) will be assessed under controlled greenhouse conditions to identify metabolites linked to sting nematode tolerance. Nematode response will be quantified through determination of root length and weight and the number of nematodes present 136 days after inoculation. Higher root length and weight indicate tolerance or resistance. Higher nematode counts indicate greater reproduction (i.e. a susceptible plant), while lower counts indicate that the accession may have some resistance. Metabolites from root tissue of these accessions will be compared to identify those associated with tolerance/resistance, and those that are associated with nematode infestation by comparing inoculated plants to uninoculated controls. Metabolomic markers will then be developed for the metabolites associated with tolerance/resistance. These markers will be used to guide future screening of bermudagrass accessions for breeding nematode-tolerant or -resistant varieties.
2015-11-13 | ST000353 | MetabolomicsWorkbench
Project description:Bermudagrass (Cynodon dactylon L.) is an important warm-season turfgrass species with well-developed stolons, which lay the foundation for fast propagation of bermudagrass plants through asexually clonal growth. However, the growth and development of bermudagrass stolons are still poorly understood at the molecular level. In this study, we comprehensively analyzed the acetylation modifications of proteins in fast growing stolons of bermudagrass cultivar Yangjiang. A total of 4657 lysine acetylation sites on 1914 proteins were successfully identified using liquid chromatography coupled to tandem mass spectrometry.
2022-08-20 | PXD034841 | Pride
Project description:Bermudagrass (Cynodon dactylon L.) is an important warm-season turfgrass species with well-developed stolons, which lay the foundation for fast propagation of bermudagrass plants through asexually clonal growth. However, the growth and development of bermudagrass stolons are still poorly understood at the molecular level. In this study, we comprehensively analyzed the succinylation modifications of proteins in fast growing stolons of bermudagrass cultivar Yangjiang. A total of 226 lysine succinylation sites on 128 proteins were successfully identified using liquid chromatography coupled to tandem mass spectrometry.
2022-08-20 | PXD034808 | Pride
Project description:Bermudagrass is an important warm-season turfgrass species with both erect growing shoots and prostrate growing stolons, however, the mechanism how bermudagrass shoots and stolons form and maintain their unique geotropic growth modes are still unclear. In this study, we compared the proteome of the internode section of shoots and stolons at the same developmental stage in bermudagrass cultivar Yangjiang. The results indicated that 376 protein species were differentially accumulated in the two types of stems.
2019-09-12 | PXD012336 | Pride
Project description:As a widely used turfgrass species, bermudagrass (Cynodon dactylon L.) can be easily propagated by colonial growth of stolons. Previous studies collectively revealed that exotic environmental factors and intrinsic hormones and genes are all involved in the differentiation, development, and diageotropically growth of stolons. However, the detailed molecular mechanism how environmental and hormone signals regulate the gene expression and biochemical activities in bermudagrass stolons remains unclear. In this study, LC-MS/MS analyses of the total protein extracts of bermudagrass stolons without preliminary phosphopeptide-enrichment successfully identified 862 nonredundant phosphorylation sites and 613 phosphoproteins.
2019-09-25 | PXD014113 | Pride
Project description:To reveal transcriptome dynamics during adventitious root formation in a coniferous tree, C. japonica, we conducted custom microarry experiments. Three parts from cuttings of easy-to-root clone of C. japonica were collected at eight time points during adventitious root formation. The results revealed major turning points on transcriptome toward adventitious root formation and the expression behavior of genes related to carbohydrate, plant hormone and others suggested the important biological changes for adventitious root formation.
2018-12-25 | GSE102874 | GEO