Ontology highlight
ABSTRACT:
PROVIDER: PRJNA646521 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:31 single-cell transcriptomes of neuroblastomas and normal human developing adrenal glands at various stages of embryonic and fetal development
| EGAD00001006624 | EGA
Project description:RNA-Seq was used to assess the gene expression profiles of 4 allodiploid embryonic stem cell lines and 4 control embryonic stem cell lines. Moreover, single-cell RNA-Seq was used to quantify the transcriptomes of 87 allodiploid single cells. Comparison of mRNA profiles of 4 allodiploid embryonic stem cell lines to 4 control mouse and rat embryonic stem cell lines using the Illumina HiSeq 2000 platform. Single-cell RNA-Seq was conducted to check the transcriptomes of single allodiploid embryonic and differentiated cells.
2016-01-31 | E-GEOD-74533 | biostudies-arrayexpress
Project description:Cellular differentiation is associated with changes in transcript populations. Accurate quantification of transcriptomes during development can thus provide global insights into differentiation processes including the fundamental specification and differentiation events operating during plant embryogenesis. However, multiple technical challenges have limited the ability to obtain high quality early embryonic transcriptomes, namely the low amount of RNA obtainable and contamination from surrounding endosperm and seed-coat tissues. We compared the performance of three low-input mRNA sequencing (mRNA-seq) library preparation kits on 0.1 to 5 nanograms (ng) of total RNA isolated from Arabidopsis thaliana (Arabidopsis) embryos and identified a low-cost method with superior performance. This mRNA-seq method was then used to profile the transcriptomes of Arabidopsis embryos across eight developmental stages. By comprehensively comparing embryonic and post-embryonic transcriptomes, we found that embryonic transcriptomes do not resemble any other plant tissue we analyzed. Moreover, transcriptome clustering analyses revealed the presence of four distinct phases of embryogenesis which are enriched in specific biological processes. We also compared zygotic embryo transcriptomes with publicly available somatic embryo transcriptomes. Strikingly, we found little resemblance between zygotic embryos and somatic embryos derived from late-staged zygotic embryos suggesting that somatic and zygotic embryo transcriptomes are distinct from each other. In addition to the biological insights gained from our systematic characterization of the Arabidopsis embryonic transcriptome, we provide a data-rich resource for the community to explore.
2018-12-21 | GSE121236 | GEO
Project description:mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis
2018-08-02 | MSV000082680 | GNPS
Project description:Comparison of the bovine embryonic transcriptomes using RNA-Seq
| PRJNA134751 | ENA
Project description:Transcriptomes during lens differentiation of human embryonic stem cells
| PRJNA348613 | ENA