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ABSTRACT: UV mutagenesis on Coprinopsis cinerea #326

PROVIDER: PRJNA648015 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12301122_1.fastq.gz	Fastqsanger.gz
	SRR12301125_1.fastq.gz	Fastqsanger.gz
	SRR12301126_1.fastq.gz	Fastqsanger.gz
	SRR12301127_1.fastq.gz	Fastqsanger.gz
	SRR12301128_1.fastq.gz	Fastqsanger.gz

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Blue light exposure and nutrient conditions influence the expression of genes involved in simultaneous hyphal knot formation in Coprinopsis cinerea.

Project description:To identify genes express in earliest stage of fruiting body initiation in Coprinopsis cinerea, strain #326 (AmutBmut haploid fruiting strain), genes expressed at 0, 0.5, 1, 3h after light exposure were compared by Super-SAGE Approximately, 1 million tags were sequenced from each mycelial sample to obtain 65,535 unique tags. First, we made matches between the tags and predicted genes of the WT strain, and 24.4% tags matched to predicted genes. Tags of 11.4% genes that did not match with predicted genes matched with the genome sequence of strain #326, and 64.1% of unique tags (2.5~4.5% of total tag counts) were not identified in the #326 genome sequence. In tags that matched to #326 strain genome, we obtained reads 1,000 bp upstream of the genome sequence from the tags, and found that 81% of 1000 bp upstream genome sequences contain predicted genes. The tag counts that matched the same predicted genes were incremented, and fisher’s exact test was carried out using IDEG6

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Project description:Genome resequencing of Coprinopsis cinerea AmutBmut pab1-1 #326

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