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Fecal Exosomes treatment of B6 mice for 14 days

ABSTRACT: Fecal Exosomes treatment of B6 mice for 14 days

PROVIDER: PRJNA659368 | ENA |

REPOSITORIES: ENA

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Fecal Exosomes treatment of B6 mice for 14 days

Project description:Fecal Exosomes from RCD and HFD (isolated from 12 months of their respective diets and purified by sucrose gradient) were orally gavaged to B6 mice for 14 days while they fed HFD. These exosomes were characterized for CD63+A33 duol positivity. After 14 days, liver tissue were harvested. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after these exosomes treatment and their link to metabolic syndrome.

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Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days [Crypt]

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Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days

Project description:Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days

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Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days [Crypt]

Project description:Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days [Crypt]

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Project description:Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days [Colon]

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Transcription profiling of mouse small intestine from wildtype B6, Rag1-/- B6 and Rag1-/- B6 mice harboring the 225.4 IgA producing hybridoma after infection with Bacteroides thetaiotaomicron

Project description:Wildtype B6, Rag1-/- B6 and Rag1-/- B6 mice harboring the 225.4 IgA producing hybridoma were colonized for 10 days with Bacteroides thetaiotaomicron Experiment Overall Design: Distal small intestine segments were snap frozen immediately after the mice were sacrificed, and small intestine divided into 16 equal segments, segments 10, 11, 12 and 14 were pooled and RNA extracted.

2008-06-17 | E-GEOD-9018 | biostudies-arrayexpress

long non coding RNA expression in post myocardial infarction 3 days and 14 days

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Sperm long RNA sequencing 14 days after dexamethasone injection

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Project description:Breast milk is the primary source of nutrition for newborns, and rich in immunological components. microRNAs (miRNAs), a well-defined group of non-coding small RNAs, are present in various body fluids (such as breast milk), which are selectively packaged inside the exosomes, a type of membrane vesicles, secreted by most cell types. These exosomal miRNAs could be actively delivered into recipient cells, and regulate target gene expression and recipient cell function. We present the lactation-related miRNA expression profiles in porcine milk exosomes across entire lactation period in pig industry (newborn to 28 days after birth) using deep sequencing technology. We found that the immune-related miRNAs are presented and enriched in breast milk exosomes, and generally resistant to relatively harsh conditions. Notably, these exosomal miRNAs exhibited the higher abundances in the colostrum (newborn to 3 days after birth) than that in the mature milk (7 to 28 days after birth), as well as in the serum of colostrum-feeding piglets compared with the only mature milk-feeding piglets. These immune-related miRNAs-loaded exosomes in breast milk may be transferred into the infant body via the digestive tract. These observations are prelude to the in-depth investigations of the essential roles of the breast milk in the development of the infant’s immune system. Eight small RNA libraries in porcine breast milk exosomes of six lactigenous stages (0, 3, 7, 14, 21 and 28 days after birth) from three female pigs were sequenced.

2012-09-17 | E-GEOD-36590 | biostudies-arrayexpress

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