Ontology highlight
ABSTRACT:
PROVIDER: PRJNA660199 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12572826.fastq.gz | Fastqsanger.gz | |||
| SRR12572827.fastq.gz | Fastqsanger.gz | |||
| SRR12572828.fastq.gz | Fastqsanger.gz | |||
| SRR12572829.fastq.gz | Fastqsanger.gz | |||
| SRR12572830.fastq.gz | Fastqsanger.gz |
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Project description:Phosphoprotein phosphatase 1 catalytic subunit gamma 2 (PPP1CC2), a PPP1 isoform restricted to testicular germ cells and spermatozoa, is essential for completion of spermatogenesis and highly similar PPP1C isoforms are capable of compensating for the loss of Ppp1cc in every tissue except in testis. In addition, inhibition of the enzymatic activity of PPP1C in spermatozoa stimulates motility. The key to understand the regulation of PPP1CC2 lies in the identification and characterization of its interacting partners. By integrating tissue-specific protein expression and protein-protein interaction data, we identified testis/sperm-enriched/specific PPP1CC complexes and potential key PPP1 interacting proteins (PIPs) for male reproduction. We constructed a testis/sperm-enriched network, analyzed several topological properties and specified the biological/physiological properties of the proteins in the network. Proteins were classified into modules according to their topological features and functional annotations, which clarified the mechanisms underlying testis and sperm function and, more specifically, the role of PPP1CC in spermatogenesis and sperm motility. Previously uncharacterized PIPs, such as AKAP4, were identified based on analyses of the interaction network. The PPP1CC2 interaction with AKAP4 in human sperm was also validated by different methods and the complex was proposed as potential targets for male contraception due to its significance in spermatozoa motility.
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Project description:This study aim to screening potential regulators of fecundity, the bovine testes of immature and mature in Chinese Red Steppes were performed by a genome-wide analysis of mRNAs and miRNAs. Compared with born testicular tissue, 6051 upregulated genes and 7104 downregulated genes in adult bovine testicular tissue were identified as differentially expressed genes (DEGs) (log2FC>1 or <－1 , FDR <0.05). The DEGs were significantly enriched in 808 GO terms (p <0.05), which are involved in sperm biological activity, including male gonad development, male genitalia development, spermatogenesis, sperm motility, spermatid development, sperm chromatin condensation, etc. Moreover, DEGs were also significantly enriched in 105 KEGG pathways (p <0.05), such as focal adhesion, cGMP-PKG signaling pathway and calcium signaling pathway, etc. To explore the miRNA-regulated gene expression network integrated analysis was used between DEGs and differentially expressed miRNAs (DERs). Correlation prediction and analysis found that 896 differentially expressed target genes were negatively correlated with the expression levels of 31 differentially expressed bovine miRNAs. Our results identified novel candidate DEGs and DERs correlated with male reproduction, which will be valuable for future genetic and epigenetic studies of sperm development and maturity, as well as provided valuable insights into the molecular mechanisms of the male fertility and spermatogenesis in cattle.
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Project description:We characterized the seminal plasma proteome of eight Beijing-you (BJY) chickens, an indigenous chicken breed in China, differ in sperm motility.
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Project description:The adverse effects of sleep disorders on male fertility are increasingly concerning. In this study, a rat model of chronic sleep restriction (CSR) was established using the modified multiplatform method. The effects of CSR on the fertility of male rats were evaluated first based on sexual behavior. Serum hormones, including testosterone (T), prolactin (PRL), luteinizing hormone (LH) and follicle-stimulating hormone (FSH), and sperm parameters (concentration, viability, motility, deformation rate) were measured, and testicular histology was analysed by hematoxylin and eosin staining. The transcriptional differences between CSR rats and control rats were detected by RNA sequencing (RNA-Seq), and DNA methylation was then detected by bisulfite sequencing. After the differently expressed genes of CSR rats were sequenced and screened, some representative up- and down-regulated genes were randomly sampled to verify the sequencing results by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Finally, functional annotations were completed, including gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomic (KEGG) pathway analyses. The results showed that the sexual behavior of CSR rats did not change when compared with control group rats. The sperm concentration, viability and motility of the CSR rats decreased significantly, while the sperm malformation rate increased significantly. In the KEGG pathway analysis database, some specific differentially expressed genes were screened, which are involved in metabolic pathways, inflammation-related pathways, the renin-angiotensin system, etc. However, the aforesaid differentially expressed genes in the testes were not related to their DNA methylation status. CSR could significantly reduce the fertility of male rats, and one of its mechanisms occurs by altering gene expression in the testes, which has nothing to do with their DNA methylation. It was suggested that CSR could cause male infertility by significantly altering the testicular transcriptome.
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