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Seminal plasma proteome of chicken with different sperm motility

ABSTRACT: We characterized the seminal plasma proteome of eight Beijing-you (BJY) chickens, an indigenous chicken breed in China, differ in sperm motility.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Gallus Gallus (chicken)

TISSUE(S): Sperm, Seminal Plasma

SUBMITTER: Jilan Chen

LAB HEAD: Jilan Chen

PROVIDER: PXD016220 | Pride | 2020-04-24

REPOSITORIES: Pride

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			Action	DRS
	20190413-LYL-L1-1.raw	Raw
	20190413-LYL-L1-2.raw	Raw
	20190413-LYL-L1-3.raw	Raw
	20190413-LYL-L1-4.raw	Raw
	20190413-LYL-L2-1.raw	Raw

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Publications

Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens.

Li Yunlei Y Sun Yanyan Y Ni Aixin A Shi Lei L Wang Panlin P Isa Adamu Mani AM Ge Pingzhuang P Jiang Linlin L Fan Jing J Ma Hui H Yang Gongshe G Chen Jilan J

Molecular & cellular proteomics : MCP 20200420 6

Molecular mechanisms underlying sperm motility have not been fully explained, particularly in chickens. The objective was to identify seminal plasma proteins associated with chicken sperm motility by comparing the seminal plasma proteomic profile of roosters with low sperm motility (LSM, <i>n</i> = 4) and high sperm motility (HSM, <i>n</i> = 4). Using a label-free MS-based method, a total of 522 seminal plasma proteins were identified, including 386 (∼74%) previously reported and 136 novel ones. ...[more]

PMID: 32312844

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Mapping proteome of seminal plasma and spermatozoa of Indian Indigenous Malnad Gidda (Bos indicus) cattle

Project description:Malnad Gidda is one among the 43 registered cattle breeds of India with unique traits and spread over Western Ghats and coastal regions of Karnataka state in India. Selection of highly elite fertile bulls for the breeding purpose is a critical control point in animal breeding programmes. Therefore, to characterize the semen proteome and to understand the semen biology of this breed, a comprehensive proteomic analysis of spermatozoa and seminal plasma has been carried out by employing SCX and bRPLC fractionation strategies in a mass-spectrometry platform. The semen samples from three Malnad Gidda bulls maintained at Southern Regional Station of ICAR-NDRI under standard managemental conditions were used in the study. The proteomic characterisation of semen from Malnad Gidda breed resulted in the identification of 5, 84,520 PSMs, from 24,467 peptides from 2,815 proteins in spermatozoa and identification of 2, 77,583 PSMs from 12,047 peptides, which resulted in 1,974 proteins from seminal plasma. Out of 2,815 proteins in spermatozoa and 1,974 proteins from seminal plasma, 969 proteins were common to both seminal plasma and spermatozoa. The biological processes and cellular localization of spermatozoa proteins were studied using DAVID tool and were further enriched the identical GO terms using REVIGO online tool. The functional enrichment analysis of identified proteins indicated their roles in the biological processes like sperm motility, spermatid development, spermatogenesis, and so on. GO studies showed the commonalities and differences in the molecular functions of the proteins exclusively identified in spermatozoa, seminal plasma and common proteins. This is the first proteomic investigation conducted on the semen samples of an Indian indigenous breed; therefore, we believe that our preliminary data should significantly advance our understanding of semen proteome of Indian cattle.

2020-06-12 | PXD014172 | Pride

Expression data from Northeastern Indigenous and Large White longissimus dorsi

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Genome-wide SNP data of Chinese indigenous horse breed (Jinjinag horse samples)

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Genome-wide SNP data of Chinese indigenous horse breed (additional horse samples)

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Affymetrix SNP array data for beak deformity and normal Beijing-You Chickens

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