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Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution

ABSTRACT: Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution

PROVIDER: PRJNA660263 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12547100_1.fastq.gz	Fastqsanger.gz
	SRR12547100_2.fastq.gz	Fastqsanger.gz
	SRR12547200_1.fastq.gz	Fastqsanger.gz
	SRR12547200_2.fastq.gz	Fastqsanger.gz
	SRR12547300_1.fastq.gz	Fastqsanger.gz

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Transcriptional and imprinting complexity in Arabidopsis seeds at single-nucleus resolution

Project description:Seeds are the basis of agriculture, yet their full transcriptional complexity has remained unknown. Here, we employ single-nucleus RNA-sequencing to characterize developing Arabidopsis thaliana seeds, with a focus on endosperm. Endosperm, the site of gene imprinting in plants, mediates the relationship between the maternal parent and embryo. We identify new cell types in the chalazal endosperm region, which interfaces with maternal tissue for nutrient unloading. We further demonstrate that the extent of parental bias of maternally expressed imprinted genes varies with cell cycle phase, and that imprinting of paternally expressed imprinted genes is strongest in chalazal endosperm. These data indicate imprinting in endosperm is heterogeneous and suggest that parental conflict, which is proposed to drive the evolution of imprinting, is fiercest at the boundary between filial and maternal tissues.

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