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RNA-seq Renin cells isolated from Ren1c-YFP mice with aortic coarctation

ABSTRACT: RNA-seq Renin cells isolated from Ren1c-YFP mice with aortic coarctation

PROVIDER: PRJNA662486 | ENA |

REPOSITORIES: ENA

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Gene expression profiles of renin producing cells in the adult kidney (captopril treated animals) isolated from Renin-YFP transgenic mice using FACS (GUDMAP Series ID:46).

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone. Renin-YFP transgenic mice were utilized to isolate renin producing cells from the kidneys of Adult mice treated with captopril, which was provided in the drinking water at a concentration of 0.5mg/ml. In addition, mice were given low-salt chow. Mice were treated for two weeks before renin producing cells were isolated from the kidney by fluorescent activated cell sorting (FACS). RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.

2012-12-05 | E-GEOD-42712 | biostudies-arrayexpress

RNA-seq Renin cells isolated from Ren1c-YFP mice with aortic coarctation

Project description:RNA-seq was performed to compare the transcriptome of renin cells to test the effects of renal perfusion pressure changes

2021-05-26 | GSE157699 | GEO

RNA-Seq As4.1 cell line and native renin cells isolated from Ren1c-YFP mice

Project description:RNA-seq was performed to compare the transcriptome of three renin cell types: juxtaglomerular cells, cells isolated from chronically recruited mice and in As4.1 cells

2020-10-31 | GSE117723 | GEO

RNA-Seq As4.1 cell line and native renin cells isolated from Ren1c-YFP mice

Project description:RNA-Seq As4.1 cell line and native renin cells isolated from Ren1c-YFP mice

| PRJNA483026 | ENA

YFP+PDGFRa+ and YFP+PDGFRa– stromal cells isolated from the small intestine of LTBRfloxedPDGFRa/YFP mice

Project description:YFP+PDGFRa+ and YFP+PDGFRa– stromal cells isolated from the small intestine of LTBRfloxedPDGFRa/YFP mice

| PRJNA824320 | ENA

YFP+ and YFP– stromal cells isolated from the small intestine of LTBRYFP mice

Project description:YFP+ and YFP– stromal cells isolated from the small intestine of LTBRYFP mice

| PRJNA824321 | ENA

YFP+ and YFP– stromal cells isolated from intestinal ulcers of indomethacin treated LTBRYFP mice

Project description:YFP+ and YFP– stromal cells isolated from intestinal ulcers of indomethacin treated LTBRYFP mice

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(Pro)renin receptor inhibition reduces plasma cholesterol and triglycerides but does not attenuate atherosclerosis in atherosclerotic mice

Project description:Purpose: to clarify the transcriptomic changes in aortic arch of inhibiting the (pro)renin receptor using (P)RR G-ASOs

2024-03-01 | GSE167972 | GEO

RNAseq of renal dendritic cells from adult Clec9a-Cre Rosa-YFP mice

Project description:We use bulk RNA sequencing of sorted cells to characterize the gene expression profiles of renal dendritic cell (DC) subsets, cDC1 and cDC2, as well as MHCII+CD64+ F4/80hi and MHCII+CD64+ CD11bhi cells. Splenic DCs and red pulp macrophages serve as reference populations for a macrophage-like or DC-like phenotype. By sorting YFP+/YFP- F4/80hi or YFP+/YFP- CD11bhi cells from Clec9a-Cre Rosa-YFP mice we aim to reveal transcriptional differences between YFP-labelled and unlabelled cells. We showed that F4/80hi cells resemble macrophages on a transcriptional level, despite their DC origin, and that renal CD11bhi cells are a transcriptionally unique subset. However, we were not able to reveal differences between YFP+ and YFP- populations.

2020-01-31 | GSE131751 | GEO

Gene expression profiles of the renin producing cells in the kidney (GUDMAP Series_id: 29)

Project description:The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing kidney. The central thesis is straightforward. The combination of fluorescent activated cell sorting (FACS) plus microarray analysis offers a powerful, efficient and effective method for the creation of a global gene expression atlas of the developing kidney. Microarrays with essentially complete genome coverage can be used to quantitate expression levels of every gene in FACS isolated components of the developing kidney. The ensuing rapid read-out provides an expression atlas that is more sensitive, more economical and more complete than would be possible by in situ hybridizations alone Renin-GFP transgenic mice were utilized to isolate renin producing cells from the kidneys of either P0 neonatal pups or Adult mice. Renin producing cells were isolated from the kidney by fluorescent activated cell sorting (FACS). RNA was isolated from FACS sorted cells and the gene expression profiles were determined by microarrays.

2010-07-16 | E-GEOD-17138 | biostudies-arrayexpress

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