Project description:Aspergillus terreus is an emerging fungal pathogen in immunocompromised patients. Due to intrinsic resistance of AmB against A. terreus and acquiring resistance to azoles, alternative antifungal strategy needs investigation. Thus, we explored the activity of phytochemicals such as Shikonin, gallic acid, coumaric acid and quercetin against A. terreus. Amongst these, shikonin showed significant inhibition at MIC50;2 µg/ml, considered for proteome profiling.
Project description:The filamentous fungus Aspergillus terreus is known to produce both industrially and pharmaceutically important secondary metabolites. The objective of this study is to investigate the effect of exogenously added butyrolactone I (BI) on the submerged culture of A. terreus, especially on the possible regulation of the secondary metabolism on the transcriptional level. In order to elucidate the presumed regulative role of butyrolactone I, a large-scale microarray gene expression study was designed and conducted with an industrially utilised A. terreus strain MUCL38669. A. terreus MUCL38669 was cultured in secondary metabolism inducing submerged conditions for nine days, where butyrolactone I was added at the beginning of the growth phase (at 24 hours p.i.), in the middle of the growth phase (at 96 hours p.i.) or in the late growth phase (at 120 hours p.i.), in addition to the control culture where no exogenous butyrolactone I was added. To obtain comprehensive gene expression profiles over the whole culture time, samples were taken at six time points: 24 hours, 48 hours, 96 hours, 120 hours, 144 hours and 216 hours post inoculation.
