Project description:We investigated the expression profiles of lncRNAs and mRNAs in peripheral blood mononuclear cells (PBMCs) from subjects with 13 Phlegm-dampness constitution (PDC) and nine balanced Balanced constitution (BC). The differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs) were identified by the “limma” method. Goal was to determine whether long non-coding RNAs (lncRNAs) play a regulatory role in metabolic disease in subjects with PDC.
Project description:Transcriptional profiling of human PBMCs comparing healthy controls, patients with diabetic nephropathy and patients with ESRD. PBMCs were analyzed as they mediate inflammatory injury. Goal was to determine effects of increasing severity of diabetic nephropathy on global PBMC gene expression. Microarray analysis of PBMCs taken from patients with varying degrees of diabetic nephropathy.
Project description:To characterize the primary and recall responses to EV71 vaccines, PBMC from 19 recipients before and after vaccination with EV71 vaccine are collected and their gene expression signatures after stimulation with EV71 antigen were compared. Four-condition experiment,pre-vaccination PBMCs (stimulation vs. no stimulation with EV71 antigen) vs. post-vaccination PBMCs (stimulation vs. no stimulation with EV71 antigen)
Project description:Using high-throughput antibody microarray, through cross-sectional sample detection and verification of samples that had undergone physical changes over time, it was found that people with balanced constitution and dampness constitution in Chinese medicine showed differences in serum protein expression. The differences were expressed as the level changes of 19 proteins such as Dectin-2, Siglec-7, AIF and TACI. The research results provided the basis for the scientific expression of traditional Chinese medicine (TCM) constitution.
Project description:Genome-wide gene expression was measured in peripheral blood mononuclear cells (PBMC) from healthy humans donors, four hours post stimulation of a single or double treatment of LPS and compared to untreated control PBMCs. We show that a second treatment with LPS induces endotoxin tolerance, with a transcription profile similar to that seen during alternative polarization (M2) of mononuclear cells. Microarray processing was performed by the Genome BC Microarray Platform. Total RNA obtained from PBMCs from blood from healthy humans after single treatment with LPS, two treatments with LPS, or untreated. Comparison of LPS or LPS/LPS to untreated cells shows the gene expression pattern of endotoxin tolerance.
Project description:Comprehensively compare the transcriptional difference in PPD stimulated PBMCs from individuals with different tuberculosis infectious status: tuberculosis patients, latent infectious individuals and healthy controls using the microarray analysis. Two-condition experiment, PBMCs vs. PPD-PBMCs. 12 individuals: 4 TB patients, 4 latent infectious individuals and 4 healthy controls.