Transcriptomic Analysis of Wheat Seedling Responses to the Systemic Acquired Resistance Inducer N-Hydroxypipecolic Acid
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ABSTRACT:
PROVIDER: PRJNA671695 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12891935_1.fastq.gz | Fastqsanger.gz | |||
| SRR12891935_2.fastq.gz | Fastqsanger.gz | |||
| SRR12891936_1.fastq.gz | Fastqsanger.gz | |||
| SRR12891936_2.fastq.gz | Fastqsanger.gz | |||
| SRR12891937_1.fastq.gz | Fastqsanger.gz |
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Project description:This experiment is to assess global changes of wheat gene expression in response to N-hydroxypipecolic acid (NHP), which is an inducer of plant systemic accquired resistance. Six plates (24 seeds per plate) of wheat (Triticum aestivum) cultivar Zhongyuan 98-68 were planted under 25ºC incubator. After three days, three plates of seedlings were treated with 1 μL 1 mM NHP per seedling, called as NHP-rep 1,2,3. The other three were treated with water and used as the control group (called as water-rep 1,2,3). The coleoptiles were collected after one day after the treatment. Each plate serves as a biological replicate for its treatment group for RNA sequencing.
2021-01-30 | GSE160041 | GEO
Project description:The fungal pathogen Fusarium graminearum can cause destructive diseases on wheat, such as Fusarium head blight and Fusarium crown rot. However, a solution is still unavailable. Recently, N-hydroxypipecolic acid (NHP) was identified as a potent signaling molecule that is capable of inducing systemic acquired resistance to bacterial, oomycete, and fungal infection in several plant species. However, it is not clear whether NHP works in wheat to resist F. graminearum infection or how NHP affects wheat gene expression. In this report, we showed that pretreatment with NHP moderately increased wheat seedling resistance to F. graminearum. Using RNA sequencing, we found that 17% of wheat-expressed genes were significantly affected by NHP treatment. The genes encoding nucleotide-binding leucine-rich repeat immune receptors were significantly overrepresented in the group of genes upregulated by NHP treatment, while the genes encoding receptor-like kinases were not. Our results suggested that NHP treatment sensitizes a subset of the immune surveillance system in wheat seedlings, thereby facilitating wheat defense against F. graminearum infection.
| S-EPMC7905219 | biostudies-literature
Project description:We investigated the relationships of the two immune-regulatory plant metabolites salicylic acid (SA) and pipecolic acid (Pip) in the establishment of plant systemic acquired resistance (SAR) in Arabidopsis thaliana induced by the bacterial pathogen Pseudomonas syringae. To characterize the transcriptional SAR response, we used wild-type Col-0 plants, SA-deficient sid2 plants and Pip-deficient ald1 plants and performed RNA-sequencing analyses (Bernsdorff et al., Plant Cell, 2016). SAR establishment in the wild-type is characterized by a strong transcriptional response systemically induced in the foliage that prepares plants for future pathogen attack by pre-activating multiple stages of defense signaling. Whereas systemic Pip elevations are indispensable for SAR and necessary for virtually the whole transcriptional SAR response, a moderate but significant SA-independent component of SAR activation and SAR gene expression is revealed. Arabidopsis thaliana plants were grown individually in pots containing a mixture of soil, vermiculite and sand (8:1:1) in a controlled cultivation chamber with a 10-h day (9 AM to 7 PM; photon flux density 100 mol m-2 s-1) / 14-h night cycle and a relative humidity of 70 %. Day and night temperatures were set to 21C and 18C, respectively. Experiments were performed with 5- to 6-week-old, naive plants exhibiting a uniform appearance. To activate SAR, plants were infiltrated between 10 AM and 12 AM into three lower (1) leaves with suspensions of the bacterial pathogen Pseudomonas syringae pv. maculicola (OD600 = 0.005). Infiltration with 10 mM MgCl2 served as the mock-control treatment. Upper (2) leaves were harvested 48 h after the primary treatment for the determination of systemic gene expression by RNA-seq analyses. Three biologically independent, replicate SAR induction experiments were performed with Col-0 and sid2 plants (experimental set 1), and three other biologically independent experiments with Col-0 and ald1 plants (experimental set 2). In each SAR experiment, at least 6 upper (2) leaves from 6 different plants pre-treated in 1 leaves with Psm (MgCl2) were pooled for one biological Psm- (mock-control) replicate. In this way, 3 biologically independent, replicate samples per treatment and plant genotype were obtained within each SAR set.
2015-12-27 | E-MTAB-4151 | biostudies-arrayexpress
Project description:Systemic acquired resistance (SAR) is a long-lasting broad-spectrum plant defense mechanism that is induced by mobile signals generated in the primarily infected leaves. Although multiple mobile SAR signals have been proposed, how these signals are perceived in the systemic leaves is unknown. Here, we show that extracellular nicotinamide adenine dinucleotide (phosphate) (eNAD(P)) accumulates in the systemic leaves and that both eNAD(P) and its receptor, the lectin receptor kinase (LecRK), LecRK-VI.2, are required in the systemic leaves for the establishment of SAR. Moreover, the mobile signal N-hydroxypipecolic acid (NHP) induces de novo NAD(P) leakage in the systemic leaves through the respiratory burst oxidase homolog RBOHF-produced reactive oxygen species (ROS). Importantly, NHP-induced systemic immunity depends on ROS, eNAD(P), and the eNAD(P) receptor complex LecRK-VI.2/ BAK1, indicating that NHP triggers SAR through the ROS-eNAD(P)-LecRK-VI.2/BAK1 signaling pathway. Our results uncovered a long-sought-after mechanism underlying the perception of mobile SAR signals in the systemic leaves
2023-10-02 | GSE225107 | GEO
Project description:Transcriptional response of Arabidopsis thaliana in systemic acquired resistance: critical roles for pipecolic acid and salicylic acid
| PRJEB12204 | ENA
Project description:A Virus-acquired Host Cytokine Controls Systemic Aging by Antagonizing Apoptosis
| PRJNA436344 | ENA
Project description:Aging is characterized by degeneration of unique tissues. However, dissecting the interconnectedness of tissue aging remains a challenge. Here, we employ a muscle-specific DNA damage model in Drosophila to reveal secreted factors that influence systemic aging in distal tissues. Utilizing this model, we uncovered a cytokine, Diedel, that when secreted from muscle or adipose can attenuate age-related intestinal tissue degeneration by promoting proliferative homeostasis of stem cells. Diedel is both necessary and sufficient to limit tissue degeneration and extend lifespan. Secreted homologs of Diedel are also found in viruses, having been acquired from host genomes. Focusing on potential mechanistic overlap between cellular aging and viral-host cell interactions and, we found that Diedel is a functionally conserved inhibitor of apoptosis and can act as a systemic rheostat to modulate cell death during aging. These results highlight a key role for secreted antagonists of apoptosis in the systemic coordination of tissue aging.
2018-07-02 | GSE111248 | GEO
Project description:Systemic acquired resistance (SAR) is a plant defense response that provides resistance against a wide range of pathogens at the whole-plant level after a primary infection. In this study, we used a data independent acquisition (DIA) phosphoproteomics platform based on the high-resolution mass spectrometry to investigate the possible molecular mechanisms modulating protein during establishment of SAR in Arabidopsis thaliana. Then RT-qPCR and physiology assays were used to validate phosphoproteome data
2021-07-23 | PXD027512 | iProX
Project description:Recent work has provided evidence for the occurrence of N-hydroxypipecolic acid (NHP) in Arabidopsis thaliana, characterized its pathogen-inducible biosynthesis by a three-step metabolic sequence from l-lysine, and established a central role for NHP in the regulation of systemic acquired resistance. Here, we show that NHP is biosynthesized in several other plant species in response to microbial attack, generally together with its direct metabolic precursor pipecolic acid and the phenolic immune signal salicylic acid. For example, NHP accumulates locally in inoculated leaves and systemically in distant leaves of cucumber in response to Pseudomonas syringae attack, in Pseudomonas-challenged tobacco and soybean leaves, in tomato inoculated with the oomycete Phytophthora infestans, in leaves of the monocot Brachypodium distachyon infected with bacterial (Xanthomonas translucens) and fungal (Magnaporthe oryzae) pathogens, and in M. oryzae-inoculated barley. Notably, resistance assays indicate that NHP acts as a potent inducer of acquired resistance to bacterial and fungal infection in distinct monocotyledonous and dicotyledonous species. Pronounced systemic accumulation of NHP in leaf phloem sap of locally inoculated cucumber supports a function for NHP as a phloem-mobile immune signal. Our study thus generalizes the existence and function of an NHP resistance pathway in plant systemic acquired resistance.
| S-EPMC7586749 | biostudies-literature
Project description:Leaf-to-leaf, systemic immune signaling known as systemic acquired resistance (SAR) is poorly understood in monocotyledonous plants. Here, we characterize systemic immunity in barley (Hordeum vulgare) triggered after primary leaf infection with either Pseudomonas syringae pathovar japonica (Psj) or Xanthomonas translucens pathovar cerealis (Xtc). Both pathogens induced resistance in systemic, uninfected leaves against a subsequent challenge infection with Xtc. In contrast to SAR in Arabidopsis thaliana, systemic immunity in barley was not associated with NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 or the local or systemic accumulation of salicylic acid (SA). Instead, we documented a moderate local but not systemic induction of abscisic acid (ABA) after infection of leaves with Psj. In contrast to SA or its functional analog benzothiadiazole, local applications of the jasmonic acid methyl ester or ABA triggered systemic immunity to Xtc. RNA-seq analysis of local and systemic transcript accumulation revealed unique gene expression changes in response to both Psj and Xtc and a clear separation of local from systemic responses. The systemic response appeared relatively modest and quantitative RT-PCR associated systemic immunity with the local and systemic induction of two WRKY and two ETHYLENE RESPONSIVE FACTOR-like transcription factors. Systemic immunity against Xtc was further associated with transcriptional changes after a secondary/systemic Xtc challenge infection; these changes were dependent on the primary treatment. Taken together, bacteria-induced systemic immunity in barley may be mediated in part by WRKY and ERF-like transcription factors possibly facilitating transcriptional reprogramming to potentiate immunity.
2014-11-24 | E-MTAB-3063 | biostudies-arrayexpress