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Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

ABSTRACT: Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

PROVIDER: PRJNA682179 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13183000.fastq.gz	Fastqsanger.gz
	SRR13183001.fastq.gz	Fastqsanger.gz
	SRR13183002.fastq.gz	Fastqsanger.gz
	SRR13183003.fastq.gz	Fastqsanger.gz
	SRR13183004.fastq.gz	Fastqsanger.gz

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Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [degradome]

Project description:Soil potassium deficiency has become a global problem in agricultural production, seriously restricting crops productions and agricultural sustainable development. Identification of the microRNAs and understanding their functions in response to low K stress will be helpful for developing crop varieties with low K tolerance. Our previous study identified a low K tolerant accession XZ153 from Tibetan wild barley. In this study, small RNA and degradome analysis were performed on two barley genotypes differing in low K tolerance (XZ153, tolerant; ZD9, sensitive) to identify the miRNAs and their targets responding to low K stress. A total of 1108 miRNAs were detected in shoots of XZ153, and ZD9 at 2 d and 7 d after low K stress, and their targets were identified through bioinformatics prediction and degradome analysis. We identified 65 differentially expressed miRNAs responding to low K stress. The results also showed that miR164c, miR169h and miR395a modules could mediate TCA cycle, glycolysis pathway and pentose phosphate pathway responding to low K stress. The osa-miR166g-3p and ghr-miR482b may act as the regulators in Ca2+ signaling pathway in response to low K stress. The methionine salvage cycle involved in ethylene biosynthesis process mediated by miR396c-3p and osa-miR171e-5p might be also involved in responding to low K stress. Some miRNAs, including miR160a, miR396c and miR169h modules, which participated in photosynthesis regulation under low K stress, differed between the two barley genotypes. In conclusion, these exclusively expressed miRNAs and their targets play the crucial roles in low K tolerance.

2020-12-06 | GSE162540 | GEO

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

2020-12-06 | GSE162539 | GEO

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley

Project description:Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley

| PRJNA682173 | ENA

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [degradome]

Project description:Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [degradome]

| PRJNA682178 | ENA

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

Project description:Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley [miRNA-seq]

| PRJNA682179 | ENA

Identification of microRNAs in response to low potassium stress in the shoots of Tibetan wild barley and cultivated barley

Project description:This SuperSeries is composed of the SubSeries listed below.

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