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Response to SARS-CoV-2 infection in cornea, limbus and sclera from human donors

ABSTRACT: Response to SARS-CoV-2 infection in cornea, limbus and sclera from human donors

PROVIDER: PRJNA688734 | ENA |

REPOSITORIES: ENA

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Response to SARS-CoV-2 infection in cornea, limbus and sclera from human donors

Project description:Here we report the transcriptional response to SARS-CoV-2 in cornea, limbus and sclera from human cadaver donor tissue. All three tissues are characterized by an NFkB-mediated chemokine response to viral infection as well as type-I interferon stimulated genes.

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Bulk RNA Barcoding and sequencing (BRB-seq) of Mock, SARS-CoV, and SARS-CoV-2 infected human airway epithelial cell cultures at 33°C and 37°C

Project description:For the assessment of host response dynamics to SARS-CoV and SARS-CoV-2 infections in human airway epithelial cells at ambient temperature corresponding to the upper or lower respiratory tract. We performed a temporal transcriptome analysis on human airway epithelial cell (hAEC) cultures infected with SARS-CoV and SARS-CoV-2, as well as uninfected hAEC cultures, incubated either at 33°C or 37°C. hAEC cultures were harvested at 24, 48 72, 96 hpi and processed for Bulk RNA Barcoding and sequencing (BRB-seq), which allows a rapid and sensitive genome-wide transcriptomic analysis in a highly multiplexed manner. Transcriptome data was obtained from a total of 7 biological donors for pairwise comparisons of SARS-CoV or SARS-CoV-2 virus-infected to unexposed hAEC cultures at respective time points and temperatures.

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