Project description:The C. elegans lifespan in the presence of Bacillus licheniformis caused induction of a large number of genes associated with anti-aging activiy including beta-oxidation Inaddition, these results indicate the B. licheniformis enhances the lifespan of Caenorhabditis elegans through serotonin signaling Two-condition experiment, C. elegans with B. lichemiformis 141 or E. coli OP50 (conrol) for 24 h. For preparing the total RNA, C. elegans were exposed to 20 mg of bacterial lawn in NGN agar for 24 h.
Project description:The C. elegans lifespan in the presence of Bacillus licheniformis caused induction of a large number of genes associated with anti-aging activiy including beta-oxidation Inaddition, these results indicate the B. licheniformis enhances the lifespan of Caenorhabditis elegans through serotonin signaling
Project description:Bacillus licheniformis (B. licheniformis) is a microorganism with a wide range of probiotic properties and applications. Isolation and identification of novel strains is a major aspect of microbial research. Besides, the role of different carbon sources affects B. licheniformis in regulating micro-environment and the mechanisms need to be further investigated. In this study, we first isolated and identified a new strain of B. licheniformis from bovine rumen fluid. Microcrystalline cellulose (MC) and cellobiose (CB) as the certain carbon sources to treat strain. Further, a combination of transcriptome and proteome analyses was used to different carbon sources effects. The results showed that B. licheniformis ABC transporter proteins, antibiotic synthesis, flagellar assembly, cellulase-related pathways and proteins were significantly up-regulated in the MC treatment compared to the CB treatment, and lactate metabolism was inhibited. In addition, MC was used as a certain carbon source to improve bacterial inhibition of B. licheniformis, its own disease resistance and to regulate the rumen micro-environment. In conclusion, our research provides a potential new probiotic for feed research and a theoretical basis for investigating the mechanisms by which bacteria respond to different carbon sources.
Project description:Tenacibaculum finnmarkense is a novel Gram-negative, aerobic bacterial strain causing skin ulcers in Atlantic salmon. This is an emerging pathogen, which may cause serious problems to aquaculture. The study was designed to compare the life stages (smolt and posmolt) and to assess effects of environment (fresh and brackis water) on the course of disease and salmon responses to the pathogen.
