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The Xa7 Resistance Gene Guards the Susceptibility Gene SWEET14 of Rice Against Exploitation by Bacterial Blight Pathogen

ABSTRACT: The Xa7 Resistance Gene Guards the Susceptibility Gene SWEET14 of Rice Against Exploitation by Bacterial Blight Pathogen

PROVIDER: PRJNA695000 | ENA |

REPOSITORIES: ENA

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The Xa7 Resistance Gene Guards the Susceptibility Gene SWEET14 of Rice Against Exploitation by Bacterial Blight Pathogen

Project description:TAL effectors of bacteria hijack host gene expression to condition disease susceptibility, while host plants counteract TAL effectors with resistance genes to thwart pathogen infections. Here we report the cloning of Xa7, TAL effector-specific expression by the essential virulence TAL effectors AvrXa7 and PthXo3, and the prevalence of Xa7 in indica rice cultivars.

2021-01-27 | GSE165583 | GEO

The <i>Xa7</i> resistance gene guards the rice susceptibility gene <i>SWEET14</i> against exploitation by the bacterial blight pathogen.

Project description:Many plant disease resistance (R) genes function specifically in reaction to the presence of cognate effectors from a pathogen. Xanthomonas oryzae pathovar oryzae (Xoo) uses transcription activator-like effectors (TALes) to target specific rice genes for expression, thereby promoting host susceptibility to bacterial blight. Here, we report the molecular characterization of Xa7, the cognate R gene to the TALes AvrXa7 and PthXo3, which target the rice major susceptibility gene SWEET14. Xa7 was mapped to a unique 74-kb region. Gene expression analysis of the region revealed a candidate gene that contained a putative AvrXa7 effector binding element (EBE) in its promoter and encoded a 113-amino-acid peptide of unknown function. Genome editing at the Xa7 locus rendered the plants susceptible to avrXa7-carrying Xoo strains. Both AvrXa7 and PthXo3 activated a GUS reporter gene fused with the EBE-containing Xa7 promoter in Nicotiana benthamiana. The EBE of Xa7 is a close mimic of the EBE of SWEET14 for TALe-induced disease susceptibility. Ectopic expression of Xa7 triggers cell death in N. benthamiana. Xa7 is prevalent in indica rice accessions from 3000 rice genomes. Xa7 appears to be an adaptation that protects against pathogen exploitation of SWEET14 and disease susceptibility.

| S-EPMC8132128 | biostudies-literature

Transcriptome dynamics Associated with Resistance and Susceptibility against Fusarium Head Blight in Four Wheat Genotypes

Project description:We report differentially expressed genes in four wheat genotypes, Nyubai, Wuhan 1 and their progeny line HC374, shaw associated with resistance and susceptibility against Fusarium Head Blight (FHB). Our result indicates an early up-regulation of LRR-RKs distinct between susceptible and resistant genotypes; subsequently, different sets of genes associated with defense response were up-regulated. Differences in expression profiles among the resistant genotypes indicate genotype specific defense mechanisms. This study also showed a greater resemblance in transcriptomics of HC374 to Nyubai, consistent with their sharing of two type II FHB resistant QTLs on 3BS and 5AS, than to Wuhan 1 which carries one QTL on 2DL in common with HC374.

2018-08-09 | GSE113128 | GEO

Project description:The Xa7 Resistance Gene Guards the Susceptibility Gene SWEET14 of Rice Against Exploitation by Bacterial Blight Pathogen

| PRJNA694939 | ENA

Transcriptome dynamics Associated with Resistance and Susceptibility against Fusarium Head Blight in Four Wheat Genotypes

Project description:Transcriptome dynamics Associated with Resistance and Susceptibility against Fusarium Head Blight in Four Wheat Genotypes

| PRJNA450087 | ENA

Transcription profiling of brains from honey bees guards of different subspecies and host colony environment

Project description:Expression profiling of guards brains as a function of individual genotype (AHB/EHB) and environment (AHB/EHB host colony)

2009-07-01 | E-TABM-606 | biostudies-arrayexpress

Transcriptional profiling of indica rice cultivar IET8585 (Ajaya) infected with bacterial leaf blight pathogen (Xoo)

Project description:An indica rice cultivar IET8585 (Ajaya), resists diverse races of the Xanthomonas oryzae pv oryzae (Xoo) pathogen attack, and is often cultivated as bacterial leaf blight (blb) resistant check in India. Earlier we reported a recessive blb resistance gene mapped to the long arm of chromosome 5 in IET8585. To further understand the mechanism of recessive and durable resistance response, two indica rice genotypes namely, i) IET8585 (Ajaya), a disease resistant indica veriety from India and ii) IR24, a bacterial leaf blight disease susceptible genotype were selected for this study. We used the 22K rice Oligoarray from Agilent technologies to study the transcript profile in the leaves of the two contrasting rice genotypes under inoculated and un-inoculated conditions during seedling stage. Keywords: Bacterial leaf blight disease resistance mechanism

2007-10-29 | GSE6244 | GEO

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Project description:Xanthomonas spp. employ transcription activator-like effectors (TALEs) to promote pathogenicity by activating host susceptibility (S) genes. Cotton GhSWEET10 is an S gene targeted by a TALE in an early isolate of Xanthomonas citri pv. malvacearum (Xcm), but not by recent field Xcm isolates. To understand the pathogenicity shift in Xcm and its adaptation to cotton, we assembled the whole genome and the TALE repertoire of three recent Xcm Texas field isolates. A newly evolved TALE, Tal7b, activated different GhSWEET genes, GhSWEET14a and GhSWEET14b. Simultaneous activation of GhSWEET14a and GhSWEET14b resulted in pronounced water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identified a pectin lyase as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9-based gene editing supported the function of GhSWEETs as S genes in cotton bacterial blight and the promise of disrupting the TALE-binding site in these genes to control the disease. Collectively, our findings elucidate the rapid evolution of TALEs in Xanthomonas field isolates and highlight the virulence mechanism wherein TALEs induce multiple S genes simultaneously to promote pathogenicity.

2024-06-26 | GSE270600 | GEO

Profiling the asexual development transcriptome of the late blight pathogen Phytophthora infestans

Project description:Much of the pathogenic success of Phytophthora infestans, the potato and tomato late blight agent, relies on its ability to generate from mycelia large amounts of sporangia, which release zoospores that encyst and form infection structures. To better understand these critical stages, Affymetrix GeneChips based on 15,650 unigenes were designed and used to profile the life cycle, through an analysis of RNA from hyphae, sporangia, cleaving sporangia, motile zoospores, and germinated zoospore cysts. Keywords: Developmental study

2008-03-15 | GSE9623 | GEO

Potato Gene Expression Associated with the RB-mediated Broad-Spectrum late blight resistance

Project description:Late blight, caused by the oomycete Phytophthora infestans, is one of the most damaging potato diseases. Genetic resistance is one of the most effective means to control the destruction caused by this pathogen. Transgenic potato lines harboring a resistance gene, RB, confer broad-spectrum, rate-reducing late blight resistance. A microarray approach was used to understand what genes are manipulated in the potato background after the addition of the RB gene that contribute to the late blight resistant phenotype. Keywords: Time course, disease state analysis

2009-10-01 | GSE13341 | GEO

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