Project description:To identify transcriptional adaptations associated with increased alkane production in Nostoc punctiforme, we performed comparative transcriptomic analysis of an alkane overproduction strain. RNA-seq data identified a large number of highly up-regulated genes in the overproduction strain potentially involved in rRNA processing, mycosporine-glycine production, and synthesis of non-ribosomal peptides including nostopeptolide A. Other up-regulated genes encoding helical carotenoid proteins, stress-induced proteins, and those for microviridin synthesis were also up-regulated. The presence of several up-regulated genes or operons on multi-copy plasmids resulted in reduced alkane production, indicating possible targets for mutagenesis that might limit lipid droplet and alkane production.
2019-11-19 | GSE140625 | GEO
Project description:Microbial electrolysis cell couple to V(V) bio-reduction
Project description:We described an actinobacterial strain Tsukamurella tyrosinosolvens PS2 capable of active utilization of linear alkanes, producing bio-surfactants, and containing two alkane hydroxylation systems. Proteomics approaches have shown the significance of differentially expressed proteins responding to hydrocarbon adverse pressure, including increased expression of the stress and resistance proteins.
Project description:In this study we compare the transcriptome of the strain Dietzia sp. DQ12-45-1b grown in octacosane , hexadecane and glucose for the characterization distinct adaption mechanisms under different alkane substrates
Project description:The ability of Bradyrhizobium japonicum and B. elkanii strains to utilize alkane and aromatic sulfonates as sole sources of sulfur for growth was investigated. All of the strains tested were able to utilize alkane sulfonates, but not aromatic sulfonates for growth. Whole-genome transcriptional profiling was used to assess B. japonicum USDA 110 genes involved in growth on alkane sulfonates, as compared to growth on sulfate and cysteine. Two sets of genes, bll7007 to bll7011 and bll6449 to 6456 were highly expressed during growth with sulfate and sulfonates. These genes were predicted to encode alkanesulfonate monooxygenases and ABC transporter components. Reverse transcription-PCR (RT-PCR) analyses showed that these genes were organized in two operon-like structures and expressed as polycistronic messages. The sulfonate monooxygenase encoded by bll7010 (ssuD) complemented an E. coli mutant defective in utilization of sulfonates. The expression of many genes that were induced during growth on cysteine and taurine were under the control of the FixLJ-FixK2-FixK1 symbiotic nitrogen fixation cascade, indicating there is a novel linkage between sulfur metabolism and nitrogen fixation. Taken together, results of this study indicate that Bradyrhizobium sp. strains are metabolically diverse and likely use organosulfur compounds for growth and survival, and for legume nodulation and nitrogen fixation in soil systems. Three independent biological materials were prepared for sulfate or sulfonate supplemented cells. Total 12 arrays including dye swap were analyzed.
Project description:To extensively investigate the effect of Snf-1 on n-alkane assimilation in Y. lipolytica, we generated ∆Snf-1 strain using a homologous recombination method. The gene expression profilling analysis between wild-type and ∆Snf-1 strains were compared.
