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ABSTRACT: Gut microbiota of Litopenaeus vannamei hepatopancreas feed with different selenium sources

PROVIDER: PRJNA712937 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13920615_1.fastq.gz	Fastqsanger.gz
	SRR13920615_2.fastq.gz	Fastqsanger.gz
	SRR13920616_1.fastq.gz	Fastqsanger.gz
	SRR13920616_2.fastq.gz	Fastqsanger.gz
	SRR13920617_1.fastq.gz	Fastqsanger.gz

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Project description:In order to gain a better understanding of the impact of Vibrio parahaemolyticus infection on genetic regulation of Litopenaeus vannamei,we performed a miRNA-seq analysis in the hepatopancreas of Litopenaeus vannamei challenged with Vibrio parahaemolyticus, using the Illumina HiSeq 2500 platform.

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Project description:RNA-Seq of Litopenaeus vannamei hepatopancreas feed with different source of selenium

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Project description:RNA from different tissues of Litopenaeus vannamei was used to test the ability of the microarray to discriminate tissue-specific gene expression profiles Keywords: validation, tissue comparison RNA from circulating hemocytes, gills, hepatopancreas, or muscle (3 biological replicates each) was hybridized to the L. vannamei microarray. The number of hybridizing genes was noted and the relationships among the global profiles in each sample were determined by cluster analysis

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Project description:RNA-Seq of Litopenaeus vannamei hepatopancreas feed with different levels of selenium under low-salinity

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Project description:Vibrio parahaemolyticus is a Gram-negative bacterium commonly found in marine and estuarine environments. Acute hepatopancreatic necrosis disease (AHPND) caused by this bacterium is an ongoing problem among shrimp farming industries. V. parahaemolyticus proteins PirA and PirB have been determined to be major virulence factors that induce AHPND. In this study, Pacific white shrimp (Litopenaeus vannamei) were challenged with recombinant PirA and PirB by a reverse gavage method and then at 30 m, 1, 2, 4, and 6 h time points, the hepatopancreas of five individual shrimp were removed and placed into RNA later. We conducted RNA sequencing of the hepatopancreas samples from a no PirA/B control (n = 5) and PirA/B-treated shrimp at the different time intervals (n=5). We evaluated the different gene expression patterns between the time groups to the control with a focus on identifying differences in innate immune function.

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