Project description:This study focuses on determining resistance to different pyrethroids in populations of Triatoma dimidiata of Boyacá, Colombia. To determine the possible causes of resistance, we sequenced RNA of three populations: laboratory, field, and under pressure of lambda-cyhalothrin. Transcriptomic profile analyses of T. dimidiata revealed differentially regulated genes in both the field population and in the laboratory population under pressure from lambda-cyhalothrin. Gene enrichment analysis showed positive regulation of transcripts related to detoxifying enzymes and mitochondrial proteins, suggesting a significant role in resistance.
Project description:The reduviid bug Triatoma dimidiata is an important Chagas disease vector widely distributed along Central America. This insect has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in peridomestic areas in Central America fed human blood. Within this context, exploring the salivary compounds that overcome host haemostatic and immune responses to assist blood-feeding is of great scientific interest. Our aim is to provide a deeper insight into T. dimidiata salivary gland molecules that may contribute to its haematophagic habit. We realized T. dimidiata salivary transcriptomic analysis and complemented it through proteomics, disclosing the set complexity of 119 secreted proteins. The large-scale approach used enriches the pharmacologically active molecules database and improves our knowledge about the complexity of salivary compounds from haematophagous vectors and their biological interactions.
Project description:Salivary multiprotein complexes from Triatoma infestans, Triatoma dimidiata, Dipetalogaster maxima, Rhodnius prolixus, and Rhodnius neglectus were identified by Blue-Native-PAGE (BN-PAGE) coupled with liquid chromatography tandem mass spectrometry.
