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Effects of circALTO on gene expression of 293T cells

ABSTRACT: Effects of circALTO on gene expression of 293T cells

PROVIDER: PRJNA719386 | ENA |

REPOSITORIES: ENA

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Effects of circALTO on gene expression of 293T cells

Project description:Purpose: The goal of the study was to determine the potential effects of ALTO expression on the human transcriptome. Method: 293T cells were transfected with circALTO1 or a control plasmid, in triplicate, and mRNA profiles sequenced. Results: mRNA profiles from circALTO1 and control cells were distinct. ALTO expressing cells induced the transcription of multiple genes previoulsy implicated in polyomavirus infections.

2021-05-25 | GSE171397 | GEO

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Gene Expression Profile of 293T and 293T-Aire-expressing cells

Project description:To analyze the impact of Aire on gene expression profile in a model cell line, we used 293T cells and transfected them either with an Aire expression plasmid pCMV-Aire (where mAire is driven by CMV promoter) or with a control plasmid pCMV2B. Total RNA was extracted 48 hours post transfection, processed and used for gene expression profiling by Affymetrix. The data demonstrate that Aire has a very broad impact, effecting (upregulating and downregulating) hundreds of differents genes, however these genes differ dramatically from its targets in medullary epithelial cells. Keywords: transfection 293T cells were seeded on 10cm plates and were grown in DMEM containing 10% FBS under standard TC conditions. Next day, the cells were transfected either by pCMV-Aire (6ug/plate) or empty pCMV (6ug/plate) using Mirus reagent, according to manufacturer's instructions. Total RNA was extracted 48 hrs post transfection by Trizol protocol according to the manufacturer's instructions. 3 biological replicates were used for Aire transfection and 2 replicates for transfection with a control plasmid.

2010-10-16 | E-GEOD-24733 | biostudies-arrayexpress

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Project description:Transcriptional profiling of Human Embryonic Kidney(293T) Cells comparing control untreated 293T cells with 293T cells transfected with A) pcDNA 3.0(-) vector[Invitrogen] (Mock) and B) Expression vector pcDNA 3.0(-) containing cloned Influenza virus H5N1and H11N1-NS1 (Non-Structural1) gene.

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Homo sapiens strain:293T cells

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Gene expression data for HEK-293T cells transfected with GFP control and plant derived DNA demethylase DMEΔ

Project description:Expression profiles of human embryonic kidney (HEK)-293T cells expressing a GFP (293T-GFP) or a truncated form of Arabidopsis DEMETER (DME) 5-methylcytosine (5mC) DNA glycosylase (293T-DMEΔ) analyzed on an Affymetrix Human Genome U133 Plus 2.0 Array Platform. These array data revealed differentially expressed genes (DEGs) between the 293T-GFP cells (without direct 5mC excision activity) and 293T-DMEΔ cells (with artificially implemented direct 5mC excision activity).

2017-09-20 | GSE96863 | GEO

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