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CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function [10X Genomics]

ABSTRACT: CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function [10X Genomics]

PROVIDER: PRJNA722299 | ENA |

REPOSITORIES: ENA

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CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function [10X Genomics]

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CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function

Project description:CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function

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CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function [Smart-seq]

Project description:Using Smart-seq2 single cell sequencing we reported that plasmablasts from dengue-infected individuals separate into 4 distinct clusters with distinct function.

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CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function

Project description:Clinically important broadly reactive B cells evolve during multiple infections. The phenotype and function of B cells re-activated after secondary infection is different compared to B cells activated after a primary infection. Here we studied CD27highCD38high plasmablasts from patients with a primary or secondary dengue virus infection. Three transcriptionally and functionally distinct plasmablast clusters were identified. The largest cluster 0/1 was plasma cell-related and contained cells coding for virus serotype cross-reactive antibodies of the IgG1 isotype, consistent with memory B cell activation during an extrafollicular response. In contrast, cells in clusters 2 and 3 expressed low levels of antibody genes and high levels of genes associated with oxidative phosphorylation, EIF2 pathway and mitochondrial dysfunction. Clusters 2 and 3, which we name T cell-dependent plasmablasts, showed a transcriptional footprint of T cell help, in line with activation from either naÃƒÂ¯ve B cells or memory B cells. Our results contribute to the understanding of the parallel B cell activation events that occur in humans after natural primary and secondary infection.

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CD27hiCD38hi plasmablasts are activated B cells of mixed origin with distinct function

Project description:This SuperSeries is composed of the SubSeries listed below.

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10X genomics human and mouse test data

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CD27<sup>hi</sup>CD38<sup>hi</sup> plasmablasts are activated B cells of mixed origin with distinct function.

Project description:Clinically important broadly reactive B cells evolve during multiple infections, with B cells re-activated after secondary infection differing from B cells activated after a primary infection. Here we studied CD27highCD38high plasmablasts from patients with a primary or secondary dengue virus infection. Three transcriptionally and functionally distinct clusters were identified. The largest cluster 0/1 was plasma cell-related, with cells coding for serotype cross-reactive antibodies of the IgG1 isotype, consistent with memory B cell activation during an extrafollicular response. Cells in clusters 2 and 3 expressed low levels of antibody genes and high levels of genes associated with oxidative phosphorylation, EIF2 pathway, and mitochondrial dysfunction. Clusters 2 and 3 showed a transcriptional footprint of T cell help, in line with activation from naive B cells or memory B cells. Our results contribute to the understanding of the parallel B cell activation events that occur in humans after natural primary and secondary infection.

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