Ontology highlight
ABSTRACT:
PROVIDER: PRJNA722304 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Mixed Conifer Forest, Northern Arizona, soil qSIP Raw sequence reads
| PRJNA649802 | ENA
Project description:Coccidioides spp. are fungal pathogens endemic to Southern California, Arizona, and other desert regions where they cause a spectrum of symptoms known as Valley Fever in otherwise healthy individuals. In the soil, Coccidioides grows in a hyphal form that produces vegetative spores (arthroconidia). When arthroconidia are inhaled by mammals they undergo an elaborate developmental transition consisting of germination, multiple rounds of nuclear division, and segmentation to form large spherules filled with vegetative endospores. Very little is understood about the molecular basis of spherule formation. Here we characterize the role of the conserved transcription factor Ryp1 in Coccidioides spherule formation. We show that Coccidioides ryp1 mutants are unable to form mature spherules, instead arresting at an early stage of spherulation. We analyze the transcriptional profile of wild-type and Δryp1 mutant cells under hyphal and spherule-promoting conditions, thereby defining a set of hyphal- or spherule-enriched transcripts that are dependent on Ryp1 for their expression. Ryp1-dependent transcripts include key virulence factors such as SOWgp, which encodes the spherule outer wall glycoprotein. In the mouse model of coccidioidomycosis, we find that the Δryp1 mutant is completely avirulent, indicating that Ryp1-dependent pathways are essential for the ability of Coccidioides to cause disease. Vaccination of C57BL/6 mice with live Δryp1 spores does not provide any protection from lethal C. posadasii intranasal infection, consistent with our findings that the Δryp1 mutant fails to make mature spherules. Taken together, this work identifies the first transcriptional regulator that drives mature spherulation and virulence in Coccidioides.
2022-03-03 | GSE178277 | GEO
Project description:Our objective was to identify gene differentially expressed between B6 and IL-33 KO decidua, myometrium and mesometrial triangle one day prior to parturition.
2023-02-06 | GSE181904 | GEO
Project description:Pathogenic fungus that is morphologically indistinguishable from Coccidioides posadasii; both cause the disease coccidioidomycosis
| PRJNA12821 | ENA
Project description:Microbial population dynamics in a photobioreactor located in Northern Sweden
| PRJEB31036 | ENA
Project description:The use of hybrid-poplar tree plantations as a source for biofuels and biomass production in temperate regions of the Northern Hemisphere has also, unintentionally, increased forest isoprene emissions to the atmosphere. The consequences of increased isoprene emissions include higher rates of tropospheric ozone production and increases in atmospheric aerosol production. Using RNA interference (RNAi) to suppress isoprene emission in several gene insertion events of hybrid-poplars, we show that this trait, which has been assumed as a requisite for the tolerance of abiotic stress, is not required for high rates of woody biomass production, even in extremely hot and dry climates. Biomass production over four years in experimental poplar plantations in Arizona and Oregon was similar among genetic lines that emitted or did not emit significant amounts of isoprene. Lines that had substantially reduced isoprene emission rates also showed decreases in flavonol pigments, which reduce oxidative damage during extremes of abiotic stress; a pattern that would be expected to amplify metabolic dysfunction during abiotic stress. Compensatory increases in the expression of other proteomic components, however, such as those that disable superoxide and other free radicals, and the fact that most biomass is produced during the spring, prior to the hottest and driest part of the growing season, explains the apparent paradox of high biomass production with low isoprene emission. The results of this study provide optimism for designing agroforest plantations of the future that provide high rates of lignocellulose production while eliminating detrimental effects of isoprene emission on atmospheric quality.
2020-01-13 | PXD013252 | Pride
Project description:Hypsopygia costalis (gold triangle) genome assembly, ilHypCost1
| PRJEB52130 | ENA
Project description:The aims of this study were to present modifications to the annotations of the genome of C. posadasii, one of two closely related species of Coccidioides, a dimorphic fungal pathogen that causes coccidioidomycosis, also called Valley Fever. Proteins present in lysates and filtrates of in vitro grown mycelia and parasitic phase spherules from C. posadasii strain Silveira were analyzed using a GeLC-MS/MS method.
2017-11-08 | PXD006489 | Pride