Dataset Information

High productivity in hybrid-poplar trees without isoprene emission to the atmosphere

ABSTRACT: The use of hybrid-poplar tree plantations as a source for biofuels and biomass production in temperate regions of the Northern Hemisphere has also, unintentionally, increased forest isoprene emissions to the atmosphere. The consequences of increased isoprene emissions include higher rates of tropospheric ozone production and increases in atmospheric aerosol production. Using RNA interference (RNAi) to suppress isoprene emission in several gene insertion events of hybrid-poplars, we show that this trait, which has been assumed as a requisite for the tolerance of abiotic stress, is not required for high rates of woody biomass production, even in extremely hot and dry climates. Biomass production over four years in experimental poplar plantations in Arizona and Oregon was similar among genetic lines that emitted or did not emit significant amounts of isoprene. Lines that had substantially reduced isoprene emission rates also showed decreases in flavonol pigments, which reduce oxidative damage during extremes of abiotic stress; a pattern that would be expected to amplify metabolic dysfunction during abiotic stress. Compensatory increases in the expression of other proteomic components, however, such as those that disable superoxide and other free radicals, and the fact that most biomass is produced during the spring, prior to the hottest and driest part of the growing season, explains the apparent paradox of high biomass production with low isoprene emission. The results of this study provide optimism for designing agroforest plantations of the future that provide high rates of lignocellulose production while eliminating detrimental effects of isoprene emission on atmospheric quality.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Populus Trichocarpa (western Balsam Poplar) (populus Balsamifera Subsp. Trichocarpa)

TISSUE(S): Leaf

SUBMITTER: Juliane Merl-Pham

LAB HEAD: Jörg-Peter Schnitzler

PROVIDER: PXD013252 | Pride | 2020-01-13

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	FRL9474A10MA3__010-EV_Predawn_T8FRL9474A10MA3-40-338435.raw	Raw
	FRL9474A12MA3__012-Ra41_Predawn_T9FRL9474A12MA3-40-338443.raw	Raw
	FRL9474A13MA4__013-Ra41_Predawn_T10FRL9474A13MA4-40-338447.raw	Raw
	FRL9474A14MA3__014-Ra41_Predawn_T11FRL9474A14MA3-40-338451.raw	Raw
	FRL9474A15MA4__015-Ra41_Predawn_T12FRL9474A15MA4-40-338622.raw	Raw

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High productivity in hybrid-poplar plantations without isoprene emission to the atmosphere.

Monson Russell K RK Winkler Barbro B Rosenstiel Todd N TN Block Katja K Merl-Pham Juliane J Strauss Steven H SH Ault Kori K Maxfield Jason J Moore David J P DJP Trahan Nicole A NA Neice Amberly A AA Shiach Ian I Barron-Gafford Greg A GA Ibsen Peter P McCorkel Joel T JT Bernhardt Jörg J Schnitzler Joerg-Peter JP

Proceedings of the National Academy of Sciences of the United States of America 20200106 3

Hybrid-poplar tree plantations provide a source for biofuel and biomass, but they also increase forest isoprene emissions. The consequences of increased isoprene emissions include higher rates of tropospheric ozone production, increases in the lifetime of methane, and increases in atmospheric aerosol production, all of which affect the global energy budget and/or lead to the degradation of air quality. Using RNA interference (RNAi) to suppress isoprene emission, we show that this trait, which is ...[more]

PMID: 31907313

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