Project description:Investigation of the gene expression changes associated with TLR2 adjuvant (lipoteichoic acid; LTA) inhibition of allergic TH2 responses in peripheral blood mononuclear cell cultures (derived from house dust mite allergic individuals) stimulated with house dust mite extract.
Project description:Investigation of the gene expression changes associated with TLR2 adjuvant (lipoteichoic acid; LTA) inhibition of allergic TH2 responses in peripheral blood mononuclear cell cultures (derived from house dust mite allergic individuals) stimulated with house dust mite extract. Pooled samples from 5 individuals were analysed by microarray; qPCR validation was carried out in a larger cohort of 23 individuals.
Project description:Response to allergen was studied in bronchial epithelial cell line H292. Cells were cultured and subsequently exposed to House dust mite or vessel (saline); Microarray data was analysed using bioinformatics and biostastics. We find a strong response to allergen in epithelial cells Experiment Overall Design: Bronchial epithelial cell line was cultured and stimulated with house dust mite extract or diluent alone. Both conditions were performed in triplicate
Project description:Using mouse lung resident conventional CD11b+ dendritic cells (CD11b+ cDCs) in the context of house-dust mite (HDM)-driven allergic airway sensitization as a model, we aimed here to identify transcriptional events regulating the pro-Th2 activity of cDCs. We used microarray analyses to identify genes differentially expressed by lung CD11b+ conventional dendritic cells in response to house dust mite allergens in wild-type and Irf3-deficient mice
Project description:Response to allergen was studied in epithelial cells derived from allergic pantients and from healthy controls. Cells were cultured after isolation from a nasal biopsy. Cells were exposed to Housed dust mite or vessel (saline); Microarray data was analysed using bioinformatics and biostaistics. We conclude that a marked difference in basal expression and in response to hous dust mite exists Experiment Overall Design: We used 5 monotypic house dust mite allergic patients and 5 non-allergic healthy controls, the cells obtained from the biopsies were cultured in two wells, for two different conditions.
Project description:The very low density lipoprotein receptor (VLDLR) is a multi-ligand receptor that mediates pleiotropic biological processes, such as brain development. In this dataset, we include the expression data obtained from lungs from mice that had been challenged with house dust mite to induce experimental asthma or saline, as a control. These data are used to obtain genes that are differentially expressed in response to VLDLR signaling. We compared wild type and VLDLR knock out mice that had been sensitized and challenged with house dust mite or saline, as a control. There were total 16 samples with four biological relicates in each group.
Project description:The goal of this project was to identify strain-dependent expression of miRNAs in lung tissue from Collaborative Cross founder strains that were sensitized and challenged with house dust mite allergen (Der p 1).
Project description:To analyze lung miRNA signatures using a house dust mite model (HDM) of asthma. microRNA sequencing (miRNA-seq) from BALB/c mice (male and female, ages 6-8 weeks old) lung tissue was performed.
Project description:To analyze circulating miRNA signatures using a house dust mite model (HDM) of asthma. microRNA sequencing (miRNA-seq) was performed using serum from BALB/c mice (male and female, ages 6-8 weeks old).
