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Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [WGBS]

ABSTRACT: Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [WGBS]

PROVIDER: PRJNA730031 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14551619_1.fastq.gz	Fastqsanger.gz
	SRR14551619_2.fastq.gz	Fastqsanger.gz
	SRR14551620_1.fastq.gz	Fastqsanger.gz
	SRR14551620_2.fastq.gz	Fastqsanger.gz
	SRR14551621_1.fastq.gz	Fastqsanger.gz

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Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [WGBS]

Project description:The culture methods for human primordial germ cell like cells (hPGCLCs) has not been established. Here we report the culture method to expand long-term culture hPGCLCs (LTC-hPGCLCs) representing migrating morphology and early hPGC transcriptome and methylome. Key components of the culture media are stem cell factor, STO-conditioned media and absence of fetal calf serum. In this condition, these LTC-hPGCLCs were highly homogenous population without feeder cells and expanded at least 150 days. They did not express senescent phenotype such as β-galactosidase activity and shortening of telomere length at the 150-day culture, indicating that the cell might be a perpetual cell line. LTC-hPGCLCs still have potent pluripotency indicating the phenotype that the cells become human embryonic germ cells which were distinguishable from human iPS cells in strong DLK1 expression and H19 biallelic expression. In summary this simple culture method and highly homogenous LTC-hPGCLCs would be a useful resource that supports investigations on human germline cells.

2021-05-15 | GSE174484 | GEO

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [RNA-Seq]

2021-05-15 | GSE174465 | GEO

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [scRNA-seq]

2021-06-25 | GSE178885 | GEO

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition

Project description:Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition

| PRJNA730027 | ENA

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [scRNA-seq]

Project description:Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [scRNA-seq]

| PRJNA741401 | ENA

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [RNA-Seq]

Project description:Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition [RNA-Seq]

| PRJNA730019 | ENA

Expanding highly homogenous population of human primordial germ cell like cells in long-term and feeder-free culture condition

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-05-15 | GSE174485 | GEO

Bulk RNAseq anaysis of PSCs and differentiating cells under on-feeder and feeder-free cultures

Project description:Gene expression profiling of human iPSCs [201B7] under on-feeder, feeder-free [StemFit], and FGF inhibitor [100 nM PD173074] -treated cultures, and differentiating cells.

2022-03-09 | GSE195689 | GEO

Whole-genome single-cell 5mC sequencing of human primordial germ cell-like cells

Project description:In this study, we evaluated the 5mC content of single cell human primordial germ cell-like cells (hPGCLCs) generated from a male human embryonic stem cell line (UCLA2, 46XY). tilizing a strand-specific, enzymatic based method of sequencing, we compared the 5mC content of the plus strand relative to the whole chromosome. We evaluated just specified hPGCLCs, at day 4 of differentiation (D4) and then D4 hPGCLCs that were cultured on a feeder layer for additional 10 days (D4C10).

2019-10-21 | GSE138982 | GEO

Maintenance of undifferentiated mouse ES cells in suspension by the serum and feeder-free defined culture condition

Project description:Mouse embryonic stem (ES) cells are indispensable for gene targeting approaches to study gene functions and regulations, the production of animal models for human diseases, and in vitro cell differentiation studies. The proven pluripotency of ES cells is expected to allow their therapeutic use for regenerative medicine. We present here a novel suspension culture method that facilitates the proliferation of pluripotent ES cells without feeder cells. The culture medium is free of either animal-derived or synthetic serum, and contains very low amounts of peptidic or proteinaceous materials, which are favorable for therapeutic use. ES cells showed sustained proliferation in the suspension culture, and their undifferentiated state and pluripotency were experimentally verified. DNA microarray analyses showed a close relationship between the elevated expression of genes related to cell adhesions and activation of the WNT signaling pathway. We suggest that this suspension culture condition provides a better alternative to the conventional attached cell culture condition, especially for possible therapeutic use, by limiting the exposure of ES cells to feeder cells and animal products. Keywords: reference design,replicate design

2008-09-02 | GSE9022 | GEO

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