Project description:Transcriptome profiling of whole proboscis and body wall of the marine Polychaeta Glycera alba, adults, wild population (sex undiscriminated), collected from the muddy-sandy intertidal flats at W Portugal (2020). Transcriptome profiling of glandular and muscular regions of proboscis of the marine Polychaeta Hediste diversicolor, adults, wild population (sex undiscriminated), collected from the muddy-sandy intertidal flats at W Portugal (2019).
Project description:Purpose: The goal of this study is to compare endothelial small RNA transcriptome to identify the target of OASL under basal or stimulated conditions by utilizing miRNA-seq. Methods: Endothelial miRNA profilies of siCTL or siOASL transfected HUVECs were generated by illumina sequencing method, in duplicate. After sequencing, the raw sequence reads are filtered based on quality. The adapter sequences are also trimmed off the raw sequence reads. rRNA removed reads are sequentially aligned to reference genome (GRCh38) and miRNA prediction is performed by miRDeep2. Results: We identified known miRNA in species (miRDeep2) in the HUVECs transfected with siCTL or siOASL. The expression profile of mature miRNA is used to analyze differentially expressed miRNA(DE miRNA). Conclusions: Our study represents the first analysis of endothelial miRNA profiles affected by OASL knockdown with biologic replicates.
Project description:A cDNA library was constructed by Novogene (CA, USA) using a Small RNA Sample Pre Kit, and Illumina sequencing was conducted according to company workflow, using 20 million reads. Raw data were filtered for quality as determined by reads with a quality score > 5, reads containing N < 10%, no 5' primer contaminants, and reads with a 3' primer and insert tag. The 3' primer sequence was trimmed and reads with a poly A/T/G/C were removed
Project description:Extreme weather events, such as heatwaves, are becoming increasingly frequent, long-lasting, and severe as global climate change continues, shaping marine biodiversity patterns worldwide. Increased risk of overheating and mortality across major taxa have been recurrently observed, jeopardizing the sustainability of ecosystem services. Molecular responses of species, which scale up to physiological and population responses, are determinant processes that modulate species sensitivity or tolerance to extreme weather events. Here, we analysed the whole-body proteome of the intertidal ragworm Hediste diversicolor (Müller, 1776), a keystone species in estuarine ecosystems and an emergent blue bio-resource, to long-lasting heatwaves (24 ºC vs 30 ºC for 1 month). We hypothesized that this species is phenotypically plastic and thus able to acclimate to heatwaves by inducing cytoprotective pathways and modulating energy metabolism to enhance its thermal tolerance and maintain survival. To test these hypotheses, worms were exposed to control (24 ºC) and a +6 ºC increase in water temperature (30 ºC) for a month, after which they were collected for whole-body proteomic (after 28 days of exposure) analysis. Concomitantly, fatty acid analysis (after 28 days of exposure) and cumulative survival, upper thermal tolerance limits and wet weight (after 30 days of exposure) were compared between control and heatwave exposed worms.
