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Influence of IWS1 TND-interacting motifs (TIMs) on transcription elongation [PRO-seq]

ABSTRACT: Influence of IWS1 TND-interacting motifs (TIMs) on transcription elongation [PRO-seq]

PROVIDER: PRJNA745686 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15100942_1.fastq.gz	Fastqsanger.gz
	SRR15100942_2.fastq.gz	Fastqsanger.gz
	SRR15100943_1.fastq.gz	Fastqsanger.gz
	SRR15100943_2.fastq.gz	Fastqsanger.gz
	SRR15100944_1.fastq.gz	Fastqsanger.gz

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Influence of IWS1 TND-interacting motifs (TIMs) on transcription elongation [PRO-seq]

Project description:To reveal the influence of TND-interacting motifs (TIMs), which bind TFIIS N-terminal domains (TNDs), we prepared KELLY cells expressing either wild-type IWS1-FLAG or IWS1-FLAG containing disruptive mutations in the unstructured TIM3 region. Following lentiviral transduction of either WT or M3 IWS1-FLAG, we prepared PRO-seq libraries from these samples and examined elongation profiles. Analysis of elongation profiles revealed that TIM3 disruption induces pervasive defects of transcription elongation.

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Project description:To reveal the influence of TND-interacting motifs (TIMs), which bind TFIIS N-terminal domains (TNDs), we prepared KELLY cells expressing either wild-type IWS1-FLAG or IWS1-FLAG containing disruptive mutations in the unstructured TIM3 region. Following lentiviral transduction of either WT or M3 IWS1-FLAG, we prepared mRNA from these samples and performed expression profiling using RNA-seq. Analysis of differential gene expression provided markers that are differentially regulated by TIM3 of IWS1.

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