Project description:<p>The Tumor Sequencing Project (TSP) Consortium is a collaboration among participants at the Baylor College of Medicine Human Genome
Sequencing Center, the Broad Institute Genome Sequencing Platform, the Dana Farber Cancer Institute, the Memorial Sloan-Kettering Cancer
Center, the Genome Sequencing Center and Siteman Cancer Center at Washington University, the M.D. Anderson Cancer Center and the
University of Michigan Medical Center. The TSP Part A will pilot approaches to large-scale identification of genomic changes in tumors
by sequencing the exonic regions of 623 genes in 188 specimens of adenocarcinoma of the lung, as well as using high density SNP
genotyping arrays for high resolution identification of changes in chromosomal copy number.</p>
<p>The TSP Part B will pilot approaches to tumor characterization of lung adenocarcinoma samples using next-generation sequencing
technologies and benchmark those results against Part A data generated with ABI3730 instruments.</p>
Project description:<p>The Tumor Sequencing Project (TSP) Consortium is a collaboration among participants at the Baylor College of Medicine Human Genome
Sequencing Center, the Broad Institute Genome Sequencing Platform, the Dana Farber Cancer Institute, the Memorial Sloan-Kettering Cancer
Center, the Genome Sequencing Center and Siteman Cancer Center at Washington University, the M.D. Anderson Cancer Center and the
University of Michigan Medical Center. The TSP Part A will pilot approaches to large-scale identification of genomic changes in tumors
by sequencing the exonic regions of 623 genes in 188 specimens of adenocarcinoma of the lung, as well as using high density SNP
genotyping arrays for high resolution identification of changes in chromosomal copy number.</p>
<p>The TSP Part B will pilot approaches to tumor characterization of lung adenocarcinoma samples using next-generation sequencing
technologies and benchmark those results against Part A data generated with ABI3730 instruments.</p>
Project description:This is a continuation of the Chordoma Sequencing Project. All cancers arise due to somatically acquired abnormalities in DNA sequence. Systematic sequencing of cancer genomes allows acquisition of complete catalogues of all classes of somatic mutation present in cancer. These mutation catalogues will allow identification of the somatically mutated cancer genes that are operative and characterise patterns of somatic mutation that may reflect previous exogenous and endogenous mutagenic exposures. In this application, we aim to perform whole genome sequencing on 10 chordoma matched genome pairs. RNA Sequencing/Methylation and SNP6 and an additional sequencing of three cancer cell lines will be added to this work.
Project description:One lung tumor and its adjacent normal were profiled for copy-number alterations with the high-resolution Affymetrix SNP6.0 Array. The SRA ID for the high throughput sequencing project will accompany our study is SRP002045.