Single cell RNA sequencing from stroma in the E16 salivary gland
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ABSTRACT:
PROVIDER: PRJNA751935 | ENA |
REPOSITORIES: ENA
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Project description:Purpose: Use scRNA Seq to find B10stromal subpopulations and define how these subpopulations contribute to salivary gland proacinar differentiation. Method: scRNA Seq on stromal enriched cells from E16 salivary glands. Model closely related cells using PCA and UMAP regressions. Find genes that broadly and specifically define cell subpopulations. Results: The epithelium and stromal fibroblasts have multiple subpopulations. Stromal cells can be subdivided by their expression of Pdgfra, Pdgfrb, Thy1, Wnt2, and Acta2. Conclusions: Pdgfra expressing stroma specifically use BMP7 to promote proacinar differentiation.
2022-03-01 | GSE181425 | GEO
Project description:Purpose: Use scRNA Seq to find stromal subpopulations and define how these subpopulations contribute to salivary gland proacinar differentiation. Method: scRNA Seq on stromal enriched cells from E16 salivary organoids. Model closely related cells using PCA and UMAP regressions. Find genes that broadly and specifically define cell subpopulations. Results: The stromal fibroblasts have multiple subpopulations. Stromal cells can be subdivided by their expression of Pdgfra, Pdgfrb, Thy1, and Acta2. Conclusions: Organoids grown with FGF2 selects for Pdgfra expressing stroma and without FGF2 Acta2 stroma dominates.
2022-03-01 | GSE193041 | GEO
Project description:The submandibular salivary gland stroma makes up only a small portion of the total salivary gland and the stromal response to salivary gland injury has been understudied. We used single-cell RNA-sequencing (scRNAseq) to analyze which cell types are present in deligated and homeostatic salivary glands, how the cell type abundance is altered during regeneration, and how the transcriptome of those cells is being altered. This will allow us to examine which cell types are important contributors torecovery from salivary gland ductal ligation injury.
2023-07-01 | GSE235677 | GEO
Project description:The submandibular salivary gland stroma makes up only a small portion of the total salivary gland and the stromal response to salivary gland injury has been understudied. We used single-cell RNA-sequencing (scRNAseq) to analyze which cell types are present in ductal ligated and mock surgery salivary glands, how the cell type abundance is altered during injury, and how the transcriptome of those cells is being altered. This will allow us to examine which cell types are important contributors to recovery from salivary gland ductal ligation injury.
2024-01-23 | GSE253916 | GEO
Project description:The submandibular salivary gland stroma makes up only a small portion of the total salivary gland and the stromal response to salivary gland injury has been understudied. We used single-cell RNA-sequencing (scRNAseq) to analyze which cell types are present in both control and ligated samples, how the cell type abundance is altered following injury, and how the transcriptome of those cells is being altered. This will allow us to examine which cell types are important contributors to fibrosis induced by salivary gland ductal ligation injury.
2023-03-29 | GSE226640 | GEO
Project description:Single cell RNA sequencing from organoid stroma derived from E16 salivary glands.
| PRJNA794170 | ENA
Project description:Development of branching organs involves a series of events regulated by proliferation, differentiation, and intricate cell-cell communication networks. Understanding the molecular drivers for these developmental stages is instrumental to device effective regenerative therapies. Here, we use single cell RNAseq of murine submandibular gland (SMG) from developmental stages corresponding to end bud initiation (E12), branching morphogenesis (E14), cytodifferentiation (E16) and postnatal development (P1, P30, and adult) to generate an atlas of SMG development. The generated resource highlights the heterogeneity in the salivary gland epithelium throughout development and allowed us to identify putative transcription factors involved in secretory acinar cell specification that occurs around E16, including Ybx1, Eno1, Atf3, and Atf4. Clustering and trajectory inference (pseudotime) analyses of this atlas illustrate and suggest that end bud and acinar populations represent the most differentiated state relative to other populations from the same developmental stage, which was associated with a global decrease in expression of numerous transcription factors. Lastly, we identified and characterized two subsets of intercalated duct cells, a Kit+ population defined by Gfra3, and a subset characterized by Gstt1 with sexually dimorphic Smgc co-expression in females and high Serpinb11 co-expression in males. Our analysis indicates that the Gstt1+ population originates from proacinar precursors suggesting plasticity in this population. This atlas can be combined with other available resources to learn about specific cell functions and to predict mechanisms involved in development of branching organs.
2020-11-11 | GSE150327 | GEO
Project description:Salivary glands that produce and secret saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. Maintenance of diverse salivary gland cells in organoids remains problematic. Here, we established human salivary gland organoids, which is composed of multiple cellular subsets, from 3 major salivary glands, including parotid gland (PG), submandibular gland (SMG), and sublingual gland (SLG). Human salivary gland organoids expressed gland-specific genes and proteins of acinar, myoepithelial, and duct cells. Organoids were maintained in growth media (named GEM) and further underwent differentiation in differentiation media (named DAM). Our study will provide an experimental platform for the exploration of mechanisms involvled in tissue regeneration, development, or several salivary gland diseases.
2022-05-05 | GSE184526 | GEO
Project description:Salivary glands that produce and secret saliva, which is essential for lubrication, digestion, immunity, and oral homeostasis, consist of diverse cells. The long-term maintenance of diverse salivary gland cells in organoids remains problematic. Here, we established long-term murine salivary gland organoids from 3 major salivary glands, including parotid gland (PG), submandibular gland (SMG), and sublingual gland (SLG). Murine salivary gland organoids expressed gland-specific genes and proteins of acinar, myoepithelial, and duct cells. Organoids were maintained in growth media (named GEM) and further underwent differentiation in differentiation media (named DAM). Our study will provide an experimental platform for the exploration of mechanisms involvled in tissue regeneration, development, or several salivary gland diseases.
2022-05-05 | GSE184091 | GEO
Project description:Loss of Irf6 leads to disruption of branching morphogenesis and secretory acnii formation in salivary gland. To determine the differentially expressed genes in Irf6 mutant, embryonic salivary gland tissues were extracted at E14.5.
2017-07-29 | GSE102020 | GEO