Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq analysis of old versus young, male versus female, and naïve versus effector memory CD4+ T cells.

ABSTRACT: RNA-seq analysis of old versus young, male versus female, and naïve versus effector memory CD4+ T cells.

PROVIDER: PRJNA764770 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15988150.fastq.gz	Fastqsanger.gz
	SRR15988151.fastq.gz	Fastqsanger.gz
	SRR15988153.fastq.gz	Fastqsanger.gz
	SRR15988154.fastq.gz	Fastqsanger.gz
	SRR15988156.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 17

Similar Datasets

RNA-seq analysis of old versus young, male versus female, and naïve versus effector memory CD4+ T cells.

Project description:RNA-seq analysis of old versus young, male versus female, and naïve versus effector memory CD4+ T cells.

| PRJNA764770 | ENA

RNA-seq analysis of old versus young, male versus female, and naïve versus effector memory CD4+ T cells.

Project description:Impaired T cell immunity in the elderly increases mortality from infectious disease. The branching of Asparagine-linked glycans is a critical negative regulator of T cell immunity. We found that N-glycan branching increases with age in female > male, naïve > memory and CD4+ > CD8+ T cells, likely contributing to immunosenescence. To investigate the mechanisms driving these changes, we performed RNA-seq analysis on highly purified young and old naive (CD3+CD4+CD25-CD62L+CD44-) and effector memory (CD3+CD4+CD25-CD62L-CD44+) CD4+ T cells from male and female mice. Comparing young and old CD4+ TN cells, 158 and 192 differentially expressed genes (DEGs) were identified in females and males, respectively. Remarkably, only 44 of these overlapped between the sexes. There were no significant differences in gene expression of N-glycan branching enzymes or other relevant glycosylation genes. Among the 114 DEGs in female but not male CD4+ TN cells, four were within the IL-7 signaling pathway, a pathway previously implicated in regulating N-glycan branching. This included reduced expression of IL7Rα (CD127) and increased expression of Suppressor of cytokine signaling 1 (Socs1), Socs3, and Janus kinase 3 (Jak3). Other IL-7 signaling pathway genes were unchanged.

2022-01-14 | GSE184496 | GEO

TMT isobaric profiling of young versus old haematopoietic stem cells

Project description:Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of old versus young haematopoietic stem and progenitor cells. Young 1 and Young 2 are samples 126 and 128 of dataset UTH_1. Old 1 and Old 2 are samples 129 and 130 of UTH_1. Young 3 is sample 131 and Old 3 is sample 130 of dataset UTH_4.

2023-10-16 | MSV000093126 | MassIVE

Phosphatidylinositol-3-kinase p110 delta differentially restrains and directs naïve versus effector CD8+ T cell transcriptional programs Phosphatidylinositol-3-kinase p110 delta differentially restrains and directs naïve versus effector CD8+ T cell transcriptional programs

Project description:Phosphatidylinositol-3-kinase p110 delta (PI3Kp110δ) is pivotal for CD8+ T cell immune responses. To inform how PI3Kp110δ regulates CD8+ T cells, the current study focuses on PI3Kp110δ controlled transcriptional programs and reveals how PI3Kp110δ selectively induces and represses expression of key genes that create a cytotoxic T cell (CTL). The data identify differences in PI3Kp110δ regulated transcriptional programs between naïve and cytotoxic T cells including differential control of cytolytic effector molecules, costimulatory receptors and the critical inhibitory receptors CTLA4 and SLAMF6. However, common to both naïve and effector cells is PI3Kp110δ control of the production of chemokines and cytokines that orchestrate communication between the adaptive and innate immune system. The study provides a comprehensive resource for understanding how PI3Kp110δ uses multiple mechanisms dependent on Protein Kinase B/AKT, FOXO1 dependent and independent mechanisms and mitogen-activated protein kinases (MAPK) to direct CD8+ T cell fate.

2021-06-14 | PXD025046 | Pride

Phosphatidylinositol-3-kinase p110 delta differentially restrains and directs naïve versus effector CD8+ T cell transcriptional programs

2021-06-14 | PXD025061 | Pride

Effector cells derived from naïve or central memory pmel-1 CD8+ T cells

Project description:Effector cells for adoptive immunotherapy can be generated by in vitro stimulation of naïve or memory subsets of CD8+ T cells. While the characteristics of CD8+ T cell subsets are well defined, the heritable influence of those populations on their effector cell progeny is not well understood. We studied effector cells generated from naïve or central memory CD8+ T cells and found that they retained distinct gene expression signatures and developmental programs. Effector cells derived from central memory cells tended to retain their CD62L+ phenotype, but also to acquire KLRG1, an indicator of cellular senescence. In contrast, the effector cell progeny of naïve cells displayed reduced terminal differentiation, and, following infusion, they displayed greater expansion, cytokine production, and tumor destruction. These data indicate that effector cells retain a gene expression imprint conferred by their naïve or central memory progenitors, and they suggest a strategy for enhancing cancer immunotherapy.

2009-06-10 | GSE16522 | GEO

Expression profile of bcl11b-deficient NAÏVE CD8+ T cells versus wild type

Project description:Naïve CD44low CD25low CD8+ T cells from Bcl11bF/F/dLck-iCre and wild type mice at steady state were sorted at 90% purity, RNA was extracted and profile for mRNA expression to identify mRNAs differentially expressed in Bcl11b-/- naïve CD8+ T cells versus wild type. Naïve CD44low CD25low CD8+ T cells purified from Bcl11bF/F/dLck-iCre and wild type mice and investigated for mRNA expression

2014-04-22 | E-GEOD-56925 | biostudies-arrayexpress

RNA sequencing of young versus aged endothelial cells

Project description:RNA sequencing of young versus aged endothelial cells

| PRJEB37793 | ENA

Expression data for effector T cells

Project description:In this study, we examined differential gene expression in naïve human CD4+ T cells, as well as in effector Th1, Th17-negative and Th17-enriched CD4- T cell subsets. We observed a marked enrichment for increased gene expression in effector CD4+ T cells compared to naive CD4+ among immune-mediated disease oci genes. Within effector T cells, expression of disease-associated genes was increased in Th17-enriched compared to Th17-negative cells. We used microarray to examine the gene expresssion profile and level of human naïve, Th1 and effector T cell subsets. Human PBMCs were isolated and sorted to naïve, CD161-CCR6- and CD161+CCR6+ memory T cells. Naïve T cells were differentiatied to Th1 cells, and CD161-CCR6- and CD161+CCR6+ memory T cells were in vitro expanded for Th17-negative and Th17-enriched effector T cells. The gene profile was compared among naive, Th1, Th17-negative, and Th17-enriched cell subsets.

2012-05-12 | E-GEOD-32901 | biostudies-arrayexpress

Neonatal naïve CD8+ T cells have effector-like gene expression that prevents memory cell formation [RNA-seq]

Project description:Neonates are intrinsically defective at creating memory CD8+ T cells in response to infection with intracellular pathogens. Here we investigated differential of small RNAs, transcription factors, and chemokine receptors regulation in neonates as compared to adults before and during infection. We found that prior to infection, naïve cells have a different expression profile for many microRNAs, and gene targets of these microRNAs show widespread expression differences. These targets and other changes in gene expression in naïve cells result in neonatal cells that get activated more easily, express chemokine receptors that home to sites of infection, and are less protected from apoptosis during contraction. As a result, changes in neonatal naïve cells drive effector cell terminal differentiation at the expense of creating long-lived memory cells. total RNAs were sequenced from adult and neonatal CD8+ T cells before and during infection

2015-09-24 | E-GEOD-65922 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data