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Single-Cell Transcriptomics of Human Substantia Nigra in Parkinson's Disease

ABSTRACT: Single-Cell Transcriptomics of Human Substantia Nigra in Parkinson's Disease

PROVIDER: PRJNA767088 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16105800.fastq.gz	Fastqsanger.gz
	SRR16106000.fastq.gz	Fastqsanger.gz
	SRR16106100.fastq.gz	Fastqsanger.gz
	SRR16105801.fastq.gz	Fastqsanger.gz
	SRR16106001.fastq.gz	Fastqsanger.gz

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Next generation sequencing reveals upregulation of the lncRNA LINC-PINT in the substantia nigra of human Parkinson's disease patients compared to control donors

Project description:Parkinson's disease (PD) is the second most common neurodegenerative disease, involving the selecive death of dopaminergic neurons in the substantia nigra pars compacta brain region. Here, we performed next generation sequencing on RNA extracted from human substantia nigra tissue. The samples, acquired from the Netherland's Brain Bank, were taken post-mortem from PD and non-PD control donors. Analysis of the data revealed an upregulation of lncRNA LINC-PINT in PD substantia nigra. LINC-PINT interracts with the Polycomb Repressive Complex 2 (PRC2), inhibiting the tracription of multiple targets. Correspondingly, analysis of the data revealed that targets of PRC2 displayed larger disease-associated differences as compared to other genes, as well as enrichment for downregulation, suggesting that the differential expression of LINC-PINT in PD effects LINC-PINT/PRC2 interaction as well.

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Transcription profiling of human substantia nigra from postmortem brain of Parkinsons disease patients (PD)

Project description:We used microarrays to detail the global program of gene expression underlying Parkinson's disease Experiment Overall Design: Substantia nigra tissue from postmortem brain of normal and Parkinson disease patients were used for RNA extraction and hybridization on Affymetrix microarrays: 9 replicates for the controls and 16 replicates for the Parkinson's disease patients were used. Both cohorts included males and females.

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Gene expression profiling of substantia nigra: a post-mortem study in Parkinson's disease and control subjects

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Transcriptomic profiling of substantia nigra and putamen in Parkinson’s disease

Project description:We report RNA sequencing results of human substantia nigra and putamen samples from Parkinson's disease patients and controls. Each substantia nigra sample is the result of pooling brain tissue from two individuals with the same , while each putamen sample is the result of pooling brain tissue from three individuals. We found 354 differentially expressed genes (DEGs) in the SN of PD patients compared to age-matched controls, while we observed 261 DEGs in the putamen samples. The top-enriched pathways from the SN were associated with “protein folding” and “neurotransmitter transport”, and the putamen DEGs with “synapse organization”. In summary, our data confirms the key role of protein folding and neuronal degeneration in the pathology of PD, and highlights new genes and pathways that have not yet been explored in the context of PD.

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Exploring transcriptome-wide changes in the brain-localized immune cells in a mouse model of Parkinson's Disease

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