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GFP CUT&Tag from P18 wild type and Zfp541-EGFP mouse testes

ABSTRACT: GFP CUT&Tag from P18 wild type and Zfp541-EGFP mouse testes

PROVIDER: PRJNA770649 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16299875_1.fastq.gz	Fastqsanger.gz
	SRR16299875_2.fastq.gz	Fastqsanger.gz
	SRR16299876_1.fastq.gz	Fastqsanger.gz
	SRR16299876_2.fastq.gz	Fastqsanger.gz
	SRR16299877_1.fastq.gz	Fastqsanger.gz

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GFP CUT&Tag from P18 wild type and Zfp541-EGFP mouse testes

Project description:GFP CUT&Tag from P18 wild type and Zfp541-EGFP mouse testes

| PRJNA770649 | ENA

GFP CUT&Tag from P18 wild type and Zfp541-EGFP mouse testes

Project description:To evaluate the chromatin binding of ZFP541 in mouse testes.

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Transcriptome analysis of meiotic prophase spermatocytes in Zfp541 +/- and Zfp541 KO mice

Project description:ZFP541 is one of the candidates for a transcriptional regulator during mouse meiotic prophase. In order to address the role of ZFP541, transcriptomes of meiotic prophase-enriched population were compared between Zfp541 +/- and Zfp541 KO testes by RNA-seq. The meiotic prophase-enriched population was isolated from Zfp541 +/- and Zfp541 KO testes at postnatal day 18 (P18), on the Rec8-3FH-GFP KI background by fluorescent sorting of GFP positive cells.

2020-12-29 | GSE163917 | GEO

Transcriptome analysis in the Zfp541 Het and Zfp541 KO testes

Project description:Transcriptome analysis in the Zfp541 Het and Zfp541 KO testes

| PRJNA770648 | ENA

Transcriptome analysis in the Zfp541 Het and Zfp541 KO testes

Project description:Due to the DNA binding affinity and interacting ability with HDACs, we wonder if ZFP541 can regulate gene expression during meiotic prophase.

2021-12-10 | GSE185740 | GEO

Identification of the interaction proteins of PRSS37 by GFP-IP/MS using testis proteins from PRSS37-EGFP knock-in mice and pAcr-EGFP transgenic mice

Project description:To identify the potential interaction partners of PRSS37, three testis lysates of Prss37E/+ mice (PRSS37-EGFP knock-in heterozygous mice) and three testis lysates of pAcr-EGFP transgenic mice were immunoprecipitated using anti-GFP mAb magnetic beads. The GFP-IP products of six samples from two groups were subsequently analyzed by mass spectrometry (MS) to identify differentially immmunoprecipitated proteins (DIPs) in the PRSS37-EGFP group. Proteins detected in pAcr-EGFP group were used as negative controls to exclude the endogenous proteins and polypeptides that interact nonspecifically with the anti-GFP mAb magnetic beads.

2021-05-06 | PXD024833 | Pride

Characterization of ZFP541's occupency sites on genome in pachytene and diplotene spermatocytes

Project description:The DSB-machinery, which induces the programmed DNA double-strand breaks (DSBs) in leptotene and zygotene stages during meiosis, needs to be kept in silence after the initiation of pachytene stage to prevent the activation of DSB checkpoint that may lead to meiotic arrest or apoptosis of germ cells. However, the mechanisms underlying this repression remain largely unknown. Here, we report that ZFP541, a germ cell-specific zinc finger protein, is responsible for the suppression of DSBs formation at late pachytene. Lack of Zfp541 in mice leads to generation of DSBs in late pachytene spermatocytes by DSB formation related-proteins and causes male infertility due to meiotic failure. Plated-based scRNA-seq of Zfp541-/- spermatocytes revealed that ZFP541 negatively regulates many meiotic prophase genes, including genes for DSB formation and their upstream transcriptional regulators, in late pachytene spermatocytes. These results were confirmed by 10x single-cell RNA-seq data on spermatogenesis of Zfp541-/- testes, which suggested that Zfp541 is required for repressing the activation of pre-pachytene gene expression programs from early to late pachytene. ZFP541 ChIP-seq on pachytene and diplotene spermatocytes demonstrated that ZFP541 occupies the promoters of meiosis initiators (e.g., Meiosin and Rxra) and a subset of their downstream genes to repress their transcription, and thus prevent the reactivation of pre-pachytene gene expression programs in pachytene spermatocytes. Thus, our results not only revealed the role of ZFP541 in maintaining the repression of pre-pachytene transcriptional programs in pachytene spermatocytes but also provide new insight into the regulation of meiotic progression by timely turning off pre-pachytene genes.

2021-04-16 | GSE172160 | GEO

Single cell RNA-seq of spermatogenesis in WT and Zfp541-KO mice (10x Chromium)

2021-04-16 | GSE172157 | GEO

Transcriptome analysis of early and late pachytene spermatocytes in WT and Zfp541-KO mice (Smart-Seq2)

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ZFP541 ChIP-seq targets during male meiotic prophase

Project description:ZFP541 is assumed to possess putative DNA-binding domains and associated with histone deacetylases, implying it plays a role in regulation of transcription via modulation of chromatin status. Therefore, we used ChIP-seq to search for targets of ZFP541 in meiotic prophase of mouse spermatocytes.

2020-12-29 | GSE163916 | GEO

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